製品の概要

  • 製品名Anti-ACF1 / BAZ1A antibody
    ACF1 / BAZ1A 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to ACF1 / BAZ1A
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment: LVSKIQVPDY YDIIKKPIAL NIIREKVNKC EYKLASEFID DIELMFSNCF EYNPRNTSEA KAGTRLQAFF HIQAQKLGLH VTPSNVDQVS TPPAAKKSRI , corresponding to amino acids 1457-1557 of Human ACF1/ BAZ1A

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • アイソタイプIgG2a
  • 軽鎖の種類kappa
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab56843 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報WB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Component of the ACF complex, an ATP-dependent chromatin remodeling complex, that regulates spacing of nucleosomes using ATP to generate evenly spaced nucleosomes along the chromatin. The ATPase activity of the complex is regulated by the length of flanking DNA. Also involved in facilitating the DNA replication process. BAZ1A is the accessory, non-catalytic subunit of the complex which can enhance and direct the process provided by the ATPase subunit, SMARCA5, probably through targeting pericentromeric heterochromatin in late S phase. Moves end-positioned nucleosomes to a predominantly central position. May have a role in nuclear receptor-mediated transcription repression.
      Component of the histone-fold protein complex CHRAC complex which faciliates nucleosome sliding by the ACF complex and enhances ACF-mediated chromatin assembly. The C-terminal regions of both CHRAC1 and POLE1 are required for these functions.
    • 組織特異性Highly expressed in testis and at low or undetectable levels in other tissues analyzed.
    • 配列類似性Belongs to the WAL family.
      Contains 1 bromo domain.
      Contains 1 DDT domain.
      Contains 1 PHD-type zinc finger.
      Contains 1 WAC domain.
    • 細胞内局在Nucleus. May target the CHRAC complex to heterochromatin.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Acf1 antibody
      • ACF1, drosophila, homolog of antibody
      • ATP dependent chromatin remodelling protein antibody
      • ATP utilizing chromatin assembly and remodeling factor 1 antibody
      • ATP-dependent chromatin-remodeling protein antibody
      • ATP-utilizing chromatin assembly and remodeling factor 1 antibody
      • B930060C03 antibody
      • Baz1a antibody
      • BAZ1A_HUMAN antibody
      • BC065123 antibody
      • Bromodomain adjacent to zinc finger domain 1A antibody
      • Bromodomain adjacent to zinc finger domain protein 1A antibody
      • cbp146 antibody
      • CHRAC subunit ACF1 antibody
      • DKFZp586E0518 antibody
      • FLJ14383 antibody
      • Gtl5 antibody
      • hACF1 antibody
      • hWALp1 antibody
      • WALp1 antibody
      • WCRF180 antibody
      • Williams syndrome transcription factor related chromatin remodeling factor 180 antibody
      • Williams syndrome transcription factor-related chromatin-remodeling factor 180 antibody
      see all

    Anti-ACF1 / BAZ1A antibody 画像

    • Lane 1: Wild-type HAP1 cell lysate (40 µg)
      Lane 2: BAZ1A knockout HAP1 cell lysate (40 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: Jurkat cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab56843 observed at 170 kDa. Red - loading control, ab176560, observed at 52 kDa.

      ab56843 was shown to recognize ACF1 / BAZ1A when ACF1 / BAZ1A knockout samples were used, along with additional cross-reactive bands. Wild-type and ACF1 / BAZ1A knockout samples were subjected to SDS-PAGE. Ab56843 and ab176560 (loading control to GAPDH) were diluted at 1 µg/ml  and 1:10,000 dilution respectively and incubated overnight at 4C. Blots were developed with IRDye® 800CW Goat anti-Mouse IgG (H + L) and IRDye® 680 Goat anti-Rabbit IgG (H + L) secondary antibodies at 1:10,000 dilution for 1 hour at room temperature before imaging.

    • Western blot against tagged recombinant protein immunogen using ab56843 ACF1 / BAZ1A antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa

    Anti-ACF1 / BAZ1A antibody (ab56843) 使用論文

    ab56843 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab56843.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"