Acetylcholinesterase Assay Kit (Fluorometric -Red) (ab138873)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 20 min
- Sample type: Cell Lysate, Plasma, Serum
- Sensitivity: 0.1 mU/ml
製品の概要
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製品名
Acetylcholinesterase Assay Kit (Fluorometric -Red)
Acetylcholinesterase キット 製品一覧 -
検出方法
Colorimetric/Fluorometric -
サンプルの種類
Serum, Plasma, Cell Lysate -
アッセイタイプ
Enzyme activity -
検出感度
0.1 mU/ml -
全工程の試験時間
0h 20m -
種交差性
交差種: Mammals, Other species -
製品の概要
Acetylcholinesterase Assay Kit (Fluorometric -Red) ( ab138873) uses a red indicator to quantify the choline produced from the hydrolysis of acetylcholine by AChE through choline oxidase-mediated enzyme coupling reactions.
The assay can be used for monitoring and quantifying AChE activity in blood, cell extracts or other solutions. The fluorescence intensity of the indicator is proportional to the amount of choline formed, which is proportional to the AChE activity.
The assay is an optimized “mix and read” assay using a simple one-step fluorometric protocol to detect as little as 0.01 mU AChE in a 100 µl assay volume (0.1 mU/ml). The assay can be performed in a 96-well or 384-well microtiter-plate format, and can be easily read with a fluorescence microplate reader at Ex/Em = 540/590 nm or with an absorbance microplate reader at OD = 575 nm.
Acetylcholinesterase assay protocol summary:
- add samples and standards to wells
- add assay mixture and incubate for 10-30 min
- analyze with microplate reader -
特記事項
Please note this product does not differentiate between acetylcholesterase (AchE) or butyrylcholinesterase (BChE) activity as both enzymes can hydrolyze acetylcholine.
If you are looking for a colorimetric kit, we recommend Acetylcholinesterase Assay Kit (Colorimetric) (ab138871). If your plate reader has a filter to measure green fluorescnce (Ex/Em = 490/520 nm), you can use Acetylcholinesterase Assay Kit (Fluorometric - Green) (ab138872).
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 200 tests AbRed Indicator 1 vial Acetylcholine 1 vial Acetylcholinesterase Probe 2 units Acetylcholinesterase Standard 1 vial Assay Buffer 1 x 10ml Dilution Buffer 1 x 10ml DMSO 1 x 100µl -
研究分野
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機能
Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis. -
組織特異性
Isoform H is highly expressed in erythrocytes. -
配列類似性
Belongs to the type-B carboxylesterase/lipase family. -
細胞内局在
Cell membrane; Cell junction > synapse. Secreted. Cell membrane and Nucleus. Only observed in apoptotic nuclei. - Information by UniProt
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別名
- ACEE
- ACES_HUMAN
- Acetylcholinesterase
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関連製品
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Related Products
画像
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AChE measured in cell lysates showing activity per 1 million cells
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AChE measured in various species showing activity per mL sample tested
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Acetylcholinesterase dose response was measured in a solid black 96-well plate with ab138873 using a fluorescence microplate reader. As low as 0.01 mU/well (0.1 mU/ml) acetylcholinesterase can be detected with 20 minutes incubation (n=3).
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab138873 は 3 報の論文で使用されています。
- Piel S et al. Succinate prodrugs in combination with atropine and pralidoxime protect cerebral mitochondrial function in a rodent model of acute organophosphate poisoning. Sci Rep 12:20329 (2022). PubMed: 36434021
- Modafferi S et al. Gene-Environment Interactions in Developmental Neurotoxicity: a Case Study of Synergy between Chlorpyrifos and CHD8 Knockout in Human BrainSpheres. Environ Health Perspect 129:77001 (2021). PubMed: 34259569
- SevIm Ç et al. In Vitro Investigation of the Effects of Imidacloprid on AChE, LDH, and GSH Levels in the L-929 Fibroblast Cell Line. Turk J Pharm Sci 17:506-510 (2020). PubMed: 33177931