Anti-Acetylcholinesterase 抗体 (ab97299)
Key features and details
- Rabbit polyclonal to Acetylcholinesterase
- Suitable for: IHC-Fr, WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Acetylcholinesterase antibody
Acetylcholinesterase 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Acetylcholinesterase -
由来種
Rabbit -
アプリケーション
適用あり: IHC-Fr, WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Cow, Cat, Monkey -
免疫原
Recombinant fragment corresponding to Human Acetylcholinesterase aa 406-610.
Database link: P22303 -
ポジティブ・コントロール
- ICC/IF: HeLa IHC-Fro: E13.5 rat brain. IHC-P: Rat fore brain and brain, mouse colon and brain. WB: Raji cells, mouse and rat brain. Mouse lung.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97299の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-Fr |
1/100 - 1/1000.
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WB |
1/500 - 1/3000. Predicted molecular weight: 68 kDa.
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ICC/IF |
1/100 - 1/200.
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IHC-P |
1/100 - 1/1000.
|
特記事項 |
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IHC-Fr
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 68 kDa. |
ICC/IF
1/100 - 1/200. |
IHC-P
1/100 - 1/1000. |
ターゲット情報
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機能
Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis. -
組織特異性
Isoform H is highly expressed in erythrocytes. -
配列類似性
Belongs to the type-B carboxylesterase/lipase family. -
細胞内局在
Cell membrane; Cell junction > synapse. Secreted. Cell membrane and Nucleus. Only observed in apoptotic nuclei. - Information by UniProt
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参照データベース
- Entrez Gene: 540446 Cow
- Entrez Gene: 43 Human
- Entrez Gene: 11423 Mouse
- Entrez Gene: 83817 Rat
- Omim: 100740 Human
- SwissProt: P23795 Cow
- SwissProt: P22303 Human
- SwissProt: P21836 Mouse
see all -
別名
- ACEE antibody
- ACES_HUMAN antibody
- Acetylcholinesterase antibody
see all
画像
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Paraffin embedded mouse brain tissue stained for AChE using ab97299 at 1/1000 dilution in immunohistochemical analysis.
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All lanes : Anti-Acetylcholinesterase antibody (ab97299) at 1/500 dilution
Lane 1 : Mouse brain
Lane 2 : Mouse lung
Lysates/proteins at 50 µg per lane.
Predicted band size: 68 kDa -
Paraffin embedded mouse colon tissue stained for AChE using ab97299 at 1/1000 dilution in immunohistochemical analysis.
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Anti-Acetylcholinesterase antibody (ab97299) at 1/500 dilution + Rat brain at 50 µg
Predicted band size: 68 kDa -
Paraffin embedded rat brain tissue stained for AChE using ab97299 at 1/1000 dilution in immunohistochemical analysis.
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Paraffin embedded rat fore brain tissue stained for AChE using ab97299 at 1/1000 dilution in immunohistochemical analysis.
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All lanes : Anti-Acetylcholinesterase antibody (ab97299) at 1/500 dilution
Lane 1 : Mouse Brain lysate
Lane 2 : Rat brain lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 68 kDa -
Frozen sectioned E13.5 rat brain tissue stained for AChE using ab97299 at 1/1000 dilution in immunohistochemical analysis.
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Anti-Acetylcholinesterase antibody (ab97299) at 1/1000 dilution + Raji whole cell lysate at 30 µg
Predicted band size: 68 kDa
7.5% SDS PAGE -
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab97299 (1) and merged with DNA probe (2) at 1/200 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab97299 は 1 報の論文で使用されています。
- Hu YT et al. Early growth response-1 regulates acetylcholinesterase and its relation with the course of Alzheimer's disease. Brain Pathol 29:502-512 (2019). PubMed: 30511454