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Chemically acetylated human histone H4
This antibody recognises a wide range of acetylated proteins.
Our Abpromise guarantee covers the use of ab193 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent dilution.|
|ELISA||Use at an assay dependent dilution.|
|ChIP||Use at an assay dependent dilution. Recommended to use 5-10µl for 25µg of chromatin.|
Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25µg of chromatin, 5µl of ab193 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.
Image courtesy of an Abreview submitted by J Stanley.ab193 used in Western Blotting.Lysate prepared from T47D cells was loaded at 100µg. Cells were pre-treated with either insulin, butyrate, resveratrol or trichostatin A. Primary antibody incubated for 24 hours at a 1/1000 dilution. An HRP-conjugated F(ab)2 secondary antibody was used at a 1/8000 dilution.