製品の概要

  • 製品名Anti-acetyl Lysine antibody
    acetyl Lysine 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to acetyl Lysine
  • アプリケーション適用あり: WB, IP, ELISA, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Species independent
  • 免疫原

    Acetylated KLH Conjugates

  • ポジティブ・コントロール
    • TSA treated mouse spleen cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPreservative: 0.1% Sodium Azide
    Constituents: Tris buffered saline
  • Concentration information loading...
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab80178 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-P 1/100. (see Abreview)

ターゲット情報

  • 関連性In the nucleus, DNA is tightly packed into nucleosomes generating an environment which is highly repressive towards DNA processes such as transcription. Acetylation of lysine residues within proteins has emerged as an important mechanism used by cells to overcome this repression. The acetylation of non-histone proteins such as transcription factors, as well as histones appears to be involved in this process. Acetylation may result in structural transitions as well as specific signaling within discrete chromatin domains. The role of acetylation in intracellular signaling has been inferred from the binding of acetylated peptides by the conserved bromodomain. Furthermore, recent findings suggest that bromodomain/acetylated-lysine recognition can serve as a regulatory mechanism in protein-protein interactions in numerous cellular processes such as chromatin remodeling and transcriptional activation. The reversible lysine acetylation of histones and non-histone proteins plays a vital role in the regulation of many cellular processes including chromatin dynamics and transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication, DNA repair, nuclear import, and neuronal repression. More than 20 acetyltransferases and 18 deacetylases have been identified so far, but the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. Over 40 transcription factors and 30 other nuclear, cytoplasmic, bacterial, and viral proteins have been shown to be acetylated in vivo.
  • 別名
    • pan acetyl Lysine antibody

Anti-acetyl Lysine antibody 画像

  • Anti-acetyl Lysine antibody (ab80178) + Cell lysates prepared from TSA treated mouse spleen cells
  • ab80178 staining acetyl Lysine in human HepG2 cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in methanol and then blocked using 2% serum for 30 minutes at 25°C. Samples were then incubated with primary antibody at 1/150 for 1 hour at 25°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 594 (red) used at a 1/1000 dilution.

    See Abreview

  • ab80178 staining acetyl Lysine in human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in paraformaldehyde and subjected to heat-mediated antigen retrieval in citric buffer, pH 6.0 prior to blocking with 10% serum for 1 hour at 20°C. The primary antibody was diluted 1/100 and incubated with the sample for 12 hour at 4°C. An HRP-conjugated goat anti-rabbit polyclonal was used as the secondary antibody, diluted 1/200.

    See Abreview

  • ab80178 staining acetyl Lysine (green) in the neuromuscular junction of fruit fly cells by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in formaldehde, permeabilized using 0.4% Triton-X, blocked with 10% NGS for 1 hour at 20°C, then incubated with ab80178 at a 1/200 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal, used at a 1/1000 dilution.

    See Abreview

Anti-acetyl Lysine antibody (ab80178) 使用論文

This product has been referenced in:
  • Escobar-Ramirez A  et al. Modification by SUMOylation Controls Both the Transcriptional Activity and the Stability of Delta-Lactoferrin. PLoS One 10:e0129965 (2015). WB ; Human . Read more (PubMed: 26090800) »
  • Miskiewicz K  et al. HDAC6 is a Bruchpilot deacetylase that facilitates neurotransmitter release. Cell Rep 8:94-102 (2014). IP . Read more (PubMed: 24981865) »

See all 4 Publications for this product

Product Wall

Thank you for your reply.

Since the antibody you bought is not from our catalogue, I cannot comment of the suitability for your planned experiments.

If this antibody is tested and guaranteed (as we do with our antibodies) for IP and...

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Thank you for your inquiry.

I am assuming that before subjecting the samples to LC-MS/MS, the enrichments of the acetylated lysine containing peptide is planned to be done with immunoprecipitation?

I am happy to confirm that we offe...

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Thank you for contacting us. We do not currently offer an Anti-acetyl Lysine antibody pre-bound to beads for IP. However, our product ab80178 Anti-acetyl Lysine antibody, a rabbit polyclonal that has been tested in IP. This could be easily bound to ...

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Thank you for your phone call yesterday and for your patience while I have been in touch with the lab regarding this antibody in IP. Unfortunately we do not have any in-house IP data, and I've looked through the references however I couldn't find much ...

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Thank you for your call today and for your questions about ab80178. I have forwarded your enquiry to the testing lab to see if they have any additional information. I will get back to you as soon as I hear from the lab. Please let me ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Fruit fly (Drosophila melanogaster) Cell (Neuromuscular junction)
Specification Neuromuscular junction
Fixative Formaldehyde
Permeabilization Yes - 0,4% Triton-X
Blocking step 0,25% BSA + 10% NGS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Oct 11 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Liver)
Specification Liver
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric buffer, pH6
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 May 28 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (human hepatoma HepG2)
Specification human hepatoma HepG2
Fixative Methanol
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Dr. Natasha Snider

Verified customer

投稿 Mar 16 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Recombinant protein (Flag-tagged Tip60)
Loading amount 0.05 µg
Specification Flag-tagged Tip60
Gel Running Conditions Reduced Denaturing (4-12% Gradient Gel)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Mar 04 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"