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Synthetic peptide: sequence surrounding the acetylated lysine 9 of histone H3
Our Abpromise guarantee covers the use of ab22550 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use a concentration of 3 µg/ml.|
|ChIP||Use a concentration of 10 µg/ml.|
|ICC/IF||1/100 - 1/500.|
|WB||1/500 - 1/2000.|
Immunoflouroescence analysis of HeLa Cells labelling lysine acetylated proteins with ab22550. Formalin fixed cells were permeabalized with 0.1& Triton X-100 in TBS for 10 mins at room temperature and subsequently blocked with BSA at room temperature for 15 mins. Cells were then probed with ab22550 at 1/100 for 1 hour at room temperature. The secondary used was a DyLight® 488 goat anti-mouse used at 1/400 for 30 minutes at room temperature. Additional counterstains used were F-actin with a DyLight® 554 Phalloidin and Neuclei stained using a Hoechst 33342 conjugate. Image was taken at X20 magnification.
Chromatin Co-Immunoprecipitation (ChIP) analysis using ab22550 binding acetylated lysines in 10E+06 LNCaP cells. Protein binding was detected using real-time PCR.
Positive control: Fold enrichment of ab22550.
Negative Control: Non-specific IgG.