Anti-Acetyl Coenzyme A Carboxylase (phospho S79) 抗体 (ab31931)
Key features and details
- Rabbit polyclonal to Acetyl Coenzyme A Carboxylase (phospho S79)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody
Acetyl Coenzyme A Carboxylase 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Acetyl Coenzyme A Carboxylase (phospho S79) -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Sheep, Goat, Chicken, Cow -
免疫原
Synthetic peptide corresponding to Rat Acetyl Coenzyme A Carboxylase aa 50-150 (phospho S79) conjugated to keyhole limpet haemocyanin.
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ポジティブ・コントロール
- RIPA lysates from mouse heart cytosol.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.184% Tris glycine, 30% Glycerol, 0.87% Sodium chloride -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab31931の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 265 kDa. Pretreating the blot with lambda phosphatase abolished antibody binding.
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特記事項 |
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WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 265 kDa. Pretreating the blot with lambda phosphatase abolished antibody binding. |
ターゲット情報
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機能
Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase. -
組織特異性
Expressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver. -
パスウェイ
Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1. -
関連疾患
Acetyl-CoA carboxylase 1 deficiency -
配列類似性
Contains 1 ATP-grasp domain.
Contains 1 biotin carboxylation domain.
Contains 1 biotinyl-binding domain.
Contains 1 carboxyltransferase domain. -
翻訳後修飾
Phosphorylation on Ser-1263 is required for interaction with BRCA1. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 396504 Chicken
- Entrez Gene: 281590 Cow
- Entrez Gene: 31 Human
- Entrez Gene: 32 Human
- Omim: 200350 Human
- Omim: 601557 Human
- SwissProt: Q9TTS3 Cow
- SwissProt: O00763 Human
see all -
別名
- ACAC antibody
- ACACA antibody
- ACACA_HUMAN antibody
see all
画像
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MCF7 cells were incubated at 37°C for 6h with vehicle control (0 µM) and different concentrations of honokiol (ab120647). Increased expression of acetyl coenzyme A carboxylase (phospho S79) (ab31931) in MCF7 cells correlates with an increase in honokiol concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab31931 at 1 µg/ml and ab8227 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.
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HepG2 cells were incubated at 37°C for 60 minutes with vehicle control (0 µM) and different concentrations of KU-55933 (ab120637). Decreased expression of Acetyl Coenzyme A Carboxylase (phospho S79) (ab31931) in HepG2 cells correlates with an increase in KU-55933 concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab31931 at 1 µg/ml and ab8227 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab31931 は 2 報の論文で使用されています。
- Chen XY et al. LB100 ameliorates nonalcoholic fatty liver disease via the AMPK/Sirt1 pathway. World J Gastroenterol 25:6607-6618 (2019). PubMed: 31832001
- Calcutt NA et al. Selective antagonism of muscarinic receptors is neuroprotective in peripheral neuropathy. J Clin Invest 127:608-622 (2017). PubMed: 28094765