Anti-Acetyl Coenzyme A Carboxylase (phospho S221) 抗体 [EPR3143Y] (ab109540)

製品の概要

  • 製品名Anti-Acetyl Coenzyme A Carboxylase (phospho S221) antibody [EPR3143Y]
    Acetyl Coenzyme A Carboxylase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR3143Y] to Acetyl Coenzyme A Carboxylase (phospho S221)
  • アプリケーション適用あり: WB, IP, IHC-P, ICCmore details
    適用なし: Flow Cyt
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Acetyl Coenzyme A Carboxylase.

  • ポジティブ・コントロール
    • HeLa cell lysate treated with calyculin A; Human muscles.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

アプリケーション

Our Abpromise guarantee covers the use of ab109540 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 277 kDa.
IP 1/10.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/100.
  • 追加情報Is unsuitable for Flow Cyt.
  • ターゲット情報

    • 機能Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase.
    • 組織特異性Expressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver.
    • パスウェイLipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.
    • 関連疾患Acetyl-CoA carboxylase 1 deficiency
    • 配列類似性Contains 1 ATP-grasp domain.
      Contains 1 biotin carboxylation domain.
      Contains 1 biotinyl-binding domain.
      Contains 1 carboxyltransferase domain.
    • 翻訳後修飾Phosphorylation on Ser-1263 is required for interaction with BRCA1.
    • 細胞内局在Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ACAC antibody
      • ACACA antibody
      • ACACA_HUMAN antibody
      • ACACB antibody
      • ACC alpha antibody
      • ACC antibody
      • ACC beta antibody
      • ACC-alpha antibody
      • ACC1 antibody
      • ACC2 antibody
      • ACCA antibody
      • ACCB antibody
      • Acetyl CoA carboxylase 1 antibody
      • Acetyl CoA carboxylase 2 antibody
      • Acetyl CoA carboxylase alpha antibody
      • Acetyl CoA carboxylase beta antibody
      • Acetyl Coenzyme A carboxylase alpha antibody
      • Acetyl Coenzyme A carboxylase beta antibody
      • Biotin carboxylase antibody
      • COA1 antibody
      • COA2 antibody
      • HACC275 antibody
      • OTTHUMP00000164069 antibody
      • OTTHUMP00000164070 antibody
      • OTTHUMP00000164076 antibody
      • OTTHUMP00000240532 antibody
      see all

    Anti-Acetyl Coenzyme A Carboxylase (phospho S221) antibody [EPR3143Y] 画像

    • All lanes : Anti-Acetyl Coenzyme A Carboxylase (phospho S221) antibody [EPR3143Y] (ab109540) at 1/1000 dilution

      Lane 1 : Untreated HeLa (human cervix adenocarcinoma) whole cell lysates
      Lane 2 : HeLa (human cervix adenocarcinoma) cells were treated with Calyculin A whole cell lysates
      Lane 3 : HeLa (human cervix adenocarcinoma) cells were treated with Calyculin A whole cell lysates. Then the membrane was incubated with Alkaline phosphatase.

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 277 kDa
      Observed band size : 265 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 minutes

      Blocking/Diluting buffer: 5% NFDM/TBST

    • ab109540, at 1/100 dilution, staining paraffin-embedded Human muscles by Immunohistochemistry.
    • All lanes : Anti-Acetyl Coenzyme A Carboxylase (phospho S221) antibody [EPR3143Y] (ab109540) at 1/5000 dilution

      Lane 1 : HeLa cell lysate, untreated
      Lane 2 : HeLa cell lysate treated with calyculin A

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 277 kDa

    Anti-Acetyl Coenzyme A Carboxylase (phospho S221) antibody [EPR3143Y] (ab109540) 使用論文

    ab109540 has not yet been referenced specifically in any publications.

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