Rabbit monoclonal [EPR3143Y] to Acetyl Coenzyme A Carboxylase (phospho S221)
The immunogen used for this product shares 100% homology with ACACB. Cross-reactivity with this protein has not been confirmed experimentally.
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Acetyl Coenzyme A Carboxylase.
HeLa cell lysate treated with calyculin A; Human muscles.
This product is a recombinant rabbit monoclonal antibody.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Predicted molecular weight: 277 kDa.
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Is unsuitable for Flow Cyt.
Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase.
Expressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver.
Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.
Acetyl-CoA carboxylase 1 deficiency
Contains 1 ATP-grasp domain.
Contains 1 biotin carboxylation domain. Contains 1 biotinyl-binding domain. Contains 1 carboxyltransferase domain.
Phosphorylation on Ser-1263 is required for interaction with BRCA1.
Information by UniProt
Western blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S221) antibody [EPR3143Y] (ab109540)
All lanes :
Anti-Acetyl Coenzyme A Carboxylase (phospho S221) antibody [EPR3143Y] (ab109540) at 1/1000 dilution
Lane 1 :
Untreated HeLa (human cervix adenocarcinoma) whole cell lysates
Lane 2 :
HeLa (human cervix adenocarcinoma) cells were treated with Calyculin A whole cell lysates
Lane 3 :
HeLa (human cervix adenocarcinoma) cells were treated with Calyculin A whole cell lysates. Then the membrane was incubated with Alkaline phosphatase.
Lysates/proteins at 10 µg per lane.
Secondary All lanes :
Goat Anti-Rabbit IgG H&L (HRP) (
) at 1/20000 dilution
Predicted band size:
Observed band size:
265 kDa (
why is the actual band size different from the predicted?
Blocking/Diluting buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Acetyl Coenzyme A Carboxylase (phospho S221) antibody [EPR3143Y] (ab109540)
ab109540, at 1/100 dilution, staining paraffin-embedded Human muscles by Immunohistochemistry.
Western blot - Acetyl Coenzyme A Carboxylase (phospho S221) antibody [EPR3143Y] (ab109540)
All lanes : Anti-Acetyl Coenzyme A Carboxylase (phospho S221) antibody [EPR3143Y] (ab109540) at 1/5000 dilution Lane 1 : HeLa cell lysate, untreated Lane 2 : HeLa cell lysate treated with calyculin A Lysates/proteins at 10 µg per lane. Secondary All lanes : HRP-labelled goat anti-rabbit at 1/2000 dilution Predicted band size: 277 kDa
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"