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RabMAb

Anti-Acetyl Coenzyme A Carboxylase 抗体 [EP687Y] (ab45174)

製品の概要

  • 製品名
    Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y]
    Acetyl Coenzyme A Carboxylase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP687Y] to Acetyl Coenzyme A Carboxylase
  • 特異性
    Acetyl Coenzyme A Carboxylase is highly expressed in lipogenic tissues such as liver, adipose, and lactating mammary gland, and its activities are regulated at various levels [Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):7515-20.].
  • アプリケーション
    適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt,ICC/IF or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues in human Acetyl Coenzyme A Carboxylase.

  • ポジティブ・コントロール
    • A431 cell lysate, human liver tissue.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

アプリケーション

Our Abpromise guarantee covers the use of ab45174 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000. Detects a band of approximately 265 kDa (predicted molecular weight: 265 kDa).Can be blocked with Acetyl Coenzyme A Carboxylase peptide (ab195232).
IHC-P Use at an assay dependent concentration.
  • 追加情報
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • ターゲット情報

    • 機能
      Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase.
    • 組織特異性
      Expressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver.
    • パスウェイ
      Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.
    • 関連疾患
      Acetyl-CoA carboxylase 1 deficiency
    • 配列類似性
      Contains 1 ATP-grasp domain.
      Contains 1 biotin carboxylation domain.
      Contains 1 biotinyl-binding domain.
      Contains 1 carboxyltransferase domain.
    • 翻訳後修飾
      Phosphorylation on Ser-1263 is required for interaction with BRCA1.
    • 細胞内局在
      Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ACAC antibody
      • ACACA antibody
      • ACACA_HUMAN antibody
      • ACACB antibody
      • ACC alpha antibody
      • ACC antibody
      • ACC beta antibody
      • ACC-alpha antibody
      • ACC1 antibody
      • ACC2 antibody
      • ACCA antibody
      • ACCB antibody
      • Acetyl CoA carboxylase 1 antibody
      • Acetyl CoA carboxylase 2 antibody
      • Acetyl CoA carboxylase alpha antibody
      • Acetyl CoA carboxylase beta antibody
      • Acetyl Coenzyme A carboxylase alpha antibody
      • Acetyl Coenzyme A carboxylase beta antibody
      • Biotin carboxylase antibody
      • COA1 antibody
      • COA2 antibody
      • HACC275 antibody
      • OTTHUMP00000164069 antibody
      • OTTHUMP00000164070 antibody
      • OTTHUMP00000164076 antibody
      • OTTHUMP00000240532 antibody
      see all

    Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y] 画像

    • Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y] (ab45174) at 1/2000 dilution + A431 cell lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP labelled, at 1/2000 dilution

      Predicted band size : 265 kDa
      Observed band size : 265 kDa
    • Ab45174, at a dilution of 1/50, staining human Acetyl Coenzyme A Carboxylase in human liver by immunohistochemistry using paraffin embedded tissue.Ab45174, at a dilution of 1/50, staining human Acetyl Coenzyme A Carboxylase in human liver by immunohistochemistry using paraffin embedded tissue.
    • Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Acetyl Coenzyme A Carboxylase with ab45174 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051, 1/500). Counter stained with hematoxylin.

       

    Anti-Acetyl Coenzyme A Carboxylase antibody [EP687Y] (ab45174) 使用論文

    This product has been referenced in:
    • Meng X  et al. Liver MicroRNA-291b-3p Promotes Hepatic Lipogenesis through Negative Regulation of Adenosine 5'-Monophosphate (AMP)-activated Protein Kinase a1. J Biol Chem 291:10625-34 (2016). Read more (PubMed: 27013659) »
    • Karlas A  et al. A human genome-wide loss-of-function screen identifies effective chikungunya antiviral drugs. Nat Commun 7:11320 (2016). Read more (PubMed: 27177310) »

    See all 8 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (C2C12 differentiated myotube)
    Gel Running Conditions
    Reduced Denaturing (4-12% Nupage Tris-Bis gel)
    Loading amount
    10 µg
    Specification
    C2C12 differentiated myotube
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    投稿 Aug 28 2017

    Thank you for your enquiry. I have contacted the originator of the product for the information you required. They have confirmed that the epitope region is indeed at 392-407 a.a. I hope this information will be helpful to you. If there is anythi...

    Read More

    Thank you for your enquiry. I can confirm that the band in the WB image is the ACC1 band. There is a 75% homology between ACC1 and ACC2. Generally, we say that if they share 85% or more, they will cross react, so I don’t think these will, but I can...

    Read More

    Thank you for your enquiry. An alignment of the peptide used to make ab45174 with human ACC2 (http://www.expasy.org/uniprot/COA2_HUMAN) shows 75% homology so there is a chance that ab45174 will cross react with ACC2. The antibody has only been test...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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