Anti-Acetyl Coenzyme A carboxylase alpha 抗体 [EPR4971] (ab109368)


  • 製品名Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR4971]
    Acetyl Coenzyme A carboxylase alpha 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4971] to Acetyl Coenzyme A carboxylase alpha
  • アプリケーション適用あり: WB, IHC-P, Flow Cyt, ICC/IFmore details
    適用なし: IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues at the N-terminus of Human Acetyl Coenzyme A carboxylase alpha

  • ポジティブ・コントロール
    • HepG2, and SH-SY5Y cell lysates. Human brain tissue and Human skeletal muscle tissue. 293T cells.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.


    Alternative versions available:

    Anti-Acetyl Coenzyme A carboxylase alpha antibody (Alexa Fluor® 488) [EPR4971] (ab203994)


    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.


Our Abpromise guarantee covers the use of ab109368 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 266 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/250.
  • 追加情報Is unsuitable for IP.
  • ターゲット情報

    • 機能Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase.
    • 組織特異性Expressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver.
    • パスウェイLipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.
    • 関連疾患Defects in ACACA are a cause of acetyl-CoA carboxylase 1 deficiency (ACACAD) [MIM:200350]; also known as ACAC deficiency or ACC deficiency. An inborn error of de novo fatty acid synthesis associated with severe brain damage, persistent myopathy and poor growth.
    • 配列類似性Contains 1 ATP-grasp domain.
      Contains 1 biotin carboxylation domain.
      Contains 1 biotinyl-binding domain.
      Contains 1 carboxyltransferase domain.
    • 翻訳後修飾Phosphorylation on Ser-1263 is required for interaction with BRCA1.
    • 細胞内局在Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ACAC antibody
      • ACACA antibody
      • ACACA_HUMAN antibody
      • ACC alpha antibody
      • ACC-alpha antibody
      • ACC1 antibody
      • ACCA antibody
      • Acetyl Coenzyme A antibody
      • Biotin carboxylase antibody
      see all

    Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR4971] 画像

    • Overlay histogram showing SH-SY5Y cells stained with ab109368 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109368, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    • All lanes : Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR4971] (ab109368) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      HRP labelled Goat anti-Rabbit at 1/2000 dilution

      Predicted band size : 266 kDa
    • Immunofluorescent staining of 293 cells using ab109368 at 1/100 dilution
    • Immunohistochemical analysis of paraffin-embedded skeletal muscle tissue using ab109368 at 1/250 dilution.
    • Immunohistochemical analysis of paraffin-embedded brain tissue using ab109368 at 1/250 dilution.

    Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR4971] (ab109368) 使用論文

    ab109368 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for participating in our 100% Abreview Testing Discount program. On17 July 2012 your review was returned with a request for any images which you may have relating to your testing of ab109368 in IHC-FoFr on mouse brain sections. We have ...

    Read More

    I am very pleased to hear you would like to accept our offer and test ab109368 in IHC on frozen mouse brain sections. This code will give you one free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for IHC ...

    Read More