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RabMAb

Anti-Acetyl Coenzyme A carboxylase alpha 抗体 [EPR4971] (ab109368)

製品の概要

  • 製品名
    Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR4971]
    Acetyl Coenzyme A carboxylase alpha 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4971] to Acetyl Coenzyme A carboxylase alpha
  • アプリケーション
    適用あり: WB, IHC-P, Flow Cyt, ICC/IFmore details
    適用なし: IP
  • 種交差性
    交差種: Human
    非交差種: Mouse, Rat
  • 免疫原

    corresponding to Human Acetyl Coenzyme A carboxylase alpha aa 1-100 (N terminal).

  • ポジティブ・コントロール
    • HepG2, and SH-SY5Y cell lysates. Human brain tissue and Human skeletal muscle tissue. 293T cells.
  • 特記事項

     Alternative versions available:

    Anti-Acetyl Coenzyme A carboxylase alpha antibody (Alexa Fluor® 488) [EPR4971] (ab203994)

    Mouse and rat:  We have preliminary internal testing data to indicate this antibody may not react with these species, Please contact us for more information. 

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109368 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/5000. Predicted molecular weight: 266 kDa.

For unpurified use at 1/1000- 1/10000.

IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/250.
  • 追加情報
    Is unsuitable for IP.
  • ターゲット情報

    • 機能
      Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase.
    • 組織特異性
      Expressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver.
    • パスウェイ
      Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.
    • 関連疾患
      Defects in ACACA are a cause of acetyl-CoA carboxylase 1 deficiency (ACACAD) [MIM:200350]; also known as ACAC deficiency or ACC deficiency. An inborn error of de novo fatty acid synthesis associated with severe brain damage, persistent myopathy and poor growth.
    • 配列類似性
      Contains 1 ATP-grasp domain.
      Contains 1 biotin carboxylation domain.
      Contains 1 biotinyl-binding domain.
      Contains 1 carboxyltransferase domain.
    • 翻訳後修飾
      Phosphorylation on Ser-1263 is required for interaction with BRCA1.
    • 細胞内局在
      Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ACAC antibody
      • ACACA antibody
      • ACACA_HUMAN antibody
      • ACC alpha antibody
      • ACC-alpha antibody
      • ACC1 antibody
      • ACCA antibody
      • Acetyl Coenzyme A antibody
      • Biotin carboxylase antibody
      see all

    Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR4971] 画像

    • Immunocytochemistry/ Immunofluorescence analysis of 293 (Human embryonic kidney epithelial cell) cells labeling Acetyl Coenzyme A carboxylase alpha with Purified ab109368 at 1:250 dilution (2.1μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    • Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Sonic Hedgehog with purified ab109368 at 1:100 dilution (5 ug/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - 90% methanol. Unlabeled control - Rabbit monoclonal IgG (Black). Cells without incubation with primary antibody and secondary antibody (Blue).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling Acetyl Coenzyme A carboxylase alpha with purified  ab109368 at 1:500 dilution (1.05 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using citrate Buffer, pH6.0. Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1:500 dilution. PBS instead of the primary antibody was used as the negative control.

    • Overlay histogram showing SH-SY5Y cells stained with unpurified ab109368 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109368, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    • All lanes : Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR4971] (ab109368) at 1/1000 dilution (unpurified)

      Lane 1 : 293T cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled Goat anti-Rabbit at 1/2000 dilution

      Predicted band size : 266 kDa
    • Immunofluorescent staining of 293 cells using unpurified ab109368 at 1/100 dilution

    • All lanes : Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR4971] (ab109368) at 1/5000 dilution

      Lane 1 : 293 (Human embryonic kidney epithelial cell) whole cell lysates
      Lane 2 : K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 266 kDa
      Observed band size : 266 kDa

      5% NFDM/TBST

    • Immunohistochemical analysis of paraffin-embedded skeletal muscle tissue using unpurified ab109368 at 1/250 dilution.

    • Immunohistochemical analysis of paraffin-embedded brain tissue using unpurified ab109368 at 1/250 dilution.

    Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR4971] (ab109368) 使用論文

    ab109368 has not yet been referenced specifically in any publications.

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    Thank you for participating in our 100% Abreview Testing Discount program. On17 July 2012 your review was returned with a request for any images which you may have relating to your testing of ab109368 in IHC-FoFr on mouse brain sections. We have ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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