Application
Immunocytochemistry/ Immunofluorescence
Sample
Fruit fly (Drosophila melanogaster) Cell (Neuromuscular junction)
Specification
Neuromuscular junction
Fixative
Formaldehyde
Permeabilization
Yes - 0,4% Triton-X
Blocking step
0,25% BSA + 10% NGS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Other product details
Dilution
1/200
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS+ 0,4% Triton + 0,25% BSA
Secondary antibody
Name
Non-Abcam antibody was used: Anti-Rabbit Secondary Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000
Additional data
Additional Notes
The image in attachment shows anti-22c10 staining (microtubule associated) (red) and Anti-acetyl Lysine staining (green) at the Drosophila neuromuscular junction
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投稿 Oct 11 2011