Application
Western blot
Sample
Mouse Cell lysate - whole cell (Hepatocyte)
Gel Running Conditions
Non-reduced Denaturing (15)
Loading amount
30 µg
Specification
Hepatocyte
Blocking step
I-Block(Applied biosystems) as blocking agent for 30 minute(s) · Concentration: 2µg/mL · Temperature: 22°C
Other product details
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking buffer
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: anti-mouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Host species: Goat
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Detection
Detection method
ECL
Exposure
4 minute(s) and 0 second(s)
Bands
Specific: 60 kDa Non-specific: 70, 60, 40, 28, 17 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Mar 11 2014