製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ACCN2
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (ELKAEEEEVG GVQPVSIQAF ASSSTLHGLA HIFSYERLSL KRALWALCFL) of Human ACCN2 (NP_064423).

  • ポジティブ・コントロール
    • HT1080 cell lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab94753 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 65 kDa. (Isoform 1) Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Also permeable for Ca(2+), Li(+) and K(+). Generates a biphasic current with a fast inactivating and a slow sustained phase. Mediates glutamate-independent Ca(2+) entry into neurons upon acidosis. This Ca(2+) overloading is toxic for cortical neurons and may be in part responsible for ischemic brain injury. Heteromeric channel assembly seems to modulate channel properties. Functions as a postsynaptic proton receptor that influences intracellular Ca(2+) concentration and calmodulin-dependent protein kinase II phosphorylation and thereby the density of dendritic spines. Modulates activity in the circuits underlying innate fear.
  • 組織特異性
    Expressed in most or all neurons.
  • 配列類似性
    Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. ACCN2 subfamily.
  • 翻訳後修飾
    Phosphorylation by PKA regulates interaction with PRKCABP and subcellular location. Phosphorylation by PKC may regulate the channel.
  • 細胞内局在
    Cell membrane. Localizes in synaptosomes at dendritic synapses of neurons. Colocalizes with DLG4.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ACCN2 antibody
    • ACCN2_HUMAN antibody
    • Acid sensing ion channel 1 antibody
    • Acid sensing ion channel 1a protein antibody
    • Acid sensing ion channel antibody
    • Acid-sensing ion channel 1 antibody
    • AI843610 antibody
    • Amiloride sensitive cation channel 2 neuronal antibody
    • Amiloride-sensitive cation channel 2 antibody
    • ASIC antibody
    • ASIC1 antibody
    • ASIC1A antibody
    • B530003N02Rik antibody
    • BNaC2 antibody
    • Brain sodium channel 2 antibody
    • Cation channel amiloride sensitive neuronal 2 antibody
    • hBNaC2 antibody
    • neuronal antibody
    see all

画像

  • Anti-ACCN2 antibody (ab94753) at 1 µg/ml (in 5% skim milk / PBS buffer) + HT1080 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 65 kDa
    Gel concentration: 12%


  • Predicted band size : 65 kDa
    Western Blot using ab94753 at 1.0ug/ml. Sample type: Hum. Adult Placenta Lysate


  • Predicted band size : 65 kDa
    Western Blot using ab94753 at 1.0ug/ml. Sample type: Hum. Fetal Heart Lysate


  • Predicted band size : 65 kDa
    Western Blot using ab94753 at 1.0ug/ml. Sample type: Hum. Fetal Liver Lysate


  • Predicted band size : 65 kDa
    Western Blot using ab94753 at 1.0ug/ml. Sample type: Hum. Fetal Lung Lysate


  • Predicted band size : 65 kDa
    Western Blot using ab94753 at 1.0ug/ml. Sample type: Hum. Fetal Muscle Lysate

プロトコール

参考文献

ab94753 has not yet been referenced specifically in any publications.

レビューと Q&A

Thank you for contacting Abcam. The ACCN2 antibody, ab94753 had not previously been tested in IF and so it is unknown as to whether this antibody would be suitable for this application or not. Based on the information that you provided, there a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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