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Recombinant fragment corresponding to Human ACBD3 aa 73-171.
Our Abpromise guarantee covers the use of ab57568 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 60 kDa.|
|Sandwich ELISA||Use at an assay dependent concentration.
Detection limit for recombinant GST tagged ACBD3 is approximately 0.1ng/ml as a capture antibody.
|IHC-P||Use a concentration of 3 µg/ml.|
|ICC/IF||Use a concentration of 10 µg/ml.|
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: ACBD3 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: HepG2 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab57568 observed at 70 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab57568 was shown to specifically react with ACBD3 when ACBD3 knockout samples were used. Wild-type and ACBD3 knockout samples were subjected to SDS-PAGE. Ab57568 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 1 µg/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Immunofluorescence of ab57568on HeLa cell (antibody concentration 10 ug/ml).
ab57568 has not yet been referenced specifically in any publications.