製品の概要

  • 製品名Anti-ACBD3 antibody [2G2]
    ACBD3 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [2G2] to ACBD3
  • アプリケーション適用あり: WB, Sandwich ELISA, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Recombinant fragment corresponding to Human ACBD3 aa 73-171.
    Sequence:

    RRLEQRWGFGLEELYGLALRFFKEKDGKAFHPTYEEKLKLVALHKQVLMG PYNPDTCPEVGFFDVLGNDRRREWAALGNMSKEDAMVEFVKLLNRCCHL


    Database link: Q9H3P7

  • ポジティブ・コントロール
    • WB: HeLa, PC-12, Raw 264 and NIH/3T3 cell lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab57568 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 60 kDa.
Sandwich ELISA Use at an assay dependent concentration.

Detection limit for recombinant GST tagged ACBD3 is approximately 0.1ng/ml as a capture antibody.

IHC-P Use a concentration of 3 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能Involved in the maintenance of Golgi structure by interacting with giantin, affecting protein transport between the endoplasmic reticulum and Golgi. Involved in hormone-induced steroid biosynthesis in testicular Leydig cells.
  • 組織特異性Ubiquitous, with highest expression in testis and ovary.
  • 配列類似性Contains 1 ACB (acyl-CoA-binding) domain.
    Contains 1 GOLD domain.
  • ドメインThe GOLD domain is essential for giantin binding.
  • 細胞内局在Golgi apparatus membrane. Mitochondrion. Also mitochondrial (via its interaction with PBR).
  • Information by UniProt
  • 参照データベース
  • 別名
    • ACBD 3 antibody
    • ACBD3 antibody
    • Acyl CoA binding domain containing protein 3 antibody
    • Acyl Coenzyme A binding domain containing 3 antibody
    • Acyl-CoA-binding domain-containing protein 3 antibody
    • GCP 60 antibody
    • GCP60 antibody
    • GCP60_HUMAN antibody
    • GOCAP 1 antibody
    • GOCAP1 antibody
    • Golgi complex associated protein 1 60kDa antibody
    • Golgi complex associated protein 1 antibody
    • Golgi complex-associated protein 1 antibody
    • Golgi phosphoprotein 1 antibody
    • Golgi resident protein GCP60 antibody
    • GOLPH 1 antibody
    • GOLPH1 antibody
    • PAP 7 antibody
    • PAP7 antibody
    • PBR and PKA associated protein 7 antibody
    • PBR associated protein antibody
    • PBR- and PKA-associated protein 7 antibody
    • Peripheral benzodiazepine receptor associated protein PAP7 antibody
    • Peripheral benzodiazepine receptor-associated protein PAP7 antibody
    • Peripherial benzodiazepine receptor associated protein antibody
    • PKA (RIalpha) associated protein antibody
    see all

Anti-ACBD3 antibody [2G2] 画像



  • Predicted band size : 60 kDa

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: ACBD3 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: HepG2 whole cell lysate (20 µg)

     

    Lanes 1 - 4: Merged signal (red and green). Green - ab57568 observed at 70 kDa. Red - loading control, ab181602, observed at 37 kDa.

     

    ab57568 was shown to specifically react with ACBD3 when ACBD3 knockout samples were used. Wild-type and ACBD3 knockout samples were subjected to SDS-PAGE. Ab57568 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 1 µg/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunofluorescence of ab57568on HeLa cell (antibody concentration 10 ug/ml).

  • ACBD3 antibody (ab57568) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human small Intestine.


  • Predicted band size : 60 kDa
    ACBD3 antibody (ab57568) at 1ug/lane + HeLa cell lysate at 25ug/lane.

Anti-ACBD3 antibody [2G2] (ab57568) 使用論文

ab57568 has not yet been referenced specifically in any publications.

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