製品の概要

  • 製品名Anti-ACAT1 antibody
    ACAT1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ACAT1
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Saccharomyces cerevisiae, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 143-192 (GHQDVMVAGG MESMSNVPYV MNRGSTPYGG VKLEDLIVKD GLTDVYNKIH) of Human ACAT1 (NP_000010).

  • ポジティブ・コントロール
    • HepG2 cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab104284 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 41 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能Plays a major role in ketone body metabolism.
  • 関連疾患Defects in ACAT1 are a cause of 3-ketothiolase deficiency (3KTD) [MIM:203750]; also known as alpha-methylacetoaceticaciduria. 3KTD is an inborn error of isoleucine catabolism characterized by intermittent ketoacidotic attacks associated with unconsciousness. Some patients die during an attack or are mentally retarded. Urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, triglylglycine, butanone is increased. It seems likely that the severity of this disease correlates better with the environmental or acquired factors than with the ACAT1 genotype.
  • 配列類似性Belongs to the thiolase family.
  • 細胞内局在Mitochondrion.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ACAT 1 antibody
    • ACAT antibody
    • acat1 antibody
    • Acetoacetyl CoA thiolase antibody
    • acetoacetyl Coenzyme A thiolase antibody
    • Acetoacetyl-CoA thiolase antibody
    • Acetyl CoA acetyltransferase, mitochondrial antibody
    • Acetyl Coenzyme A acetyltransferase 1 antibody
    • Acetyl-CoA acetyltransferase antibody
    • acetyl-coa acetyltransferase precursor, mitochondrial antibody
    • Acetyl-CoA thiolase, mitochondrial antibody
    • acetyl-Coenzyme A acetyltransferase 1 antibody
    • MAT antibody
    • mitochondrial acetoacetyl-CoA thiolase antibody
    • mitochondrial antibody
    • RATACAL antibody
    • T2 antibody
    • testicular tissue protein Li 198 antibody
    • THIL antibody
    • THIL_HUMAN antibody
    see all

Anti-ACAT1 antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human fetal heart tissue labelling ACAT1 with ab104284.

  • Anti-ACAT1 antibody (ab104284) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Predicted band size : 41 kDa
  • All lanes : Anti-ACAT1 antibody (ab104284) at 1/2000 dilution

    Lane 1 : HEK293T whole cell lysate
    Lane 2 : HepG2 whole cell lysate
    Lane 3 : Mouse embryonic fibroblast whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP-conjugated goat anti-rabbit polyclonal IgG at 1/2000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 41 kDa
    Observed band size : 41 kDa


    Exposure time : 2 minutes

    This image is courtesy of an Abreview submitted by Mr Dongil Kim

    See Abreview

Anti-ACAT1 antibody (ab104284) 使用論文

ab104284 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HepG2, 293T, MEF)
Loading amount 20 µg
Specification HepG2, 293T, MEF
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7%
Username

Mr. Dongil Kim

Verified customer

投稿 Jan 21 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"