製品の概要

  • 製品名Anti-ACAT1 antibody [9H10AB4]
    ACAT1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [9H10AB4] to ACAT1
  • アプリケーション適用あり: ICC, Flow Cyt, IP, IHC-P, In-Cell ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Human liver mitochondria

  • ポジティブ・コントロール
    • ICC: Human HDFn cells IHC-P: Human cerebellum IP: HepG2 cells and Human liver mitochondria FC: HeLa cells
  • 特記事項

    Product was previously marketed under the MitoSciences sub-brand.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab110290 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC Use a concentration of 5 µg/ml.
Flow Cyt Use a concentration of 1 µg/ml. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.
IHC-P 1/100. Perform heat mediated antigen retrieval via the pressure cooker method (1 minute, with 1 mmol EDTA at pH8) before commencing with IHC staining protocol.
In-Cell ELISA Use a concentration of 0.4 µg/ml. (0.4 µg/well)

ターゲット情報

  • 機能Plays a major role in ketone body metabolism.
  • 関連疾患Defects in ACAT1 are a cause of 3-ketothiolase deficiency (3KTD) [MIM:203750]; also known as alpha-methylacetoaceticaciduria. 3KTD is an inborn error of isoleucine catabolism characterized by intermittent ketoacidotic attacks associated with unconsciousness. Some patients die during an attack or are mentally retarded. Urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, triglylglycine, butanone is increased. It seems likely that the severity of this disease correlates better with the environmental or acquired factors than with the ACAT1 genotype.
  • 配列類似性Belongs to the thiolase family.
  • 細胞内局在Mitochondrion.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ACAT 1 antibody
    • ACAT antibody
    • acat1 antibody
    • Acetoacetyl CoA thiolase antibody
    • acetoacetyl Coenzyme A thiolase antibody
    • Acetoacetyl-CoA thiolase antibody
    • Acetyl CoA acetyltransferase, mitochondrial antibody
    • Acetyl Coenzyme A acetyltransferase 1 antibody
    • Acetyl-CoA acetyltransferase antibody
    • acetyl-coa acetyltransferase precursor, mitochondrial antibody
    • Acetyl-CoA thiolase, mitochondrial antibody
    • acetyl-Coenzyme A acetyltransferase 1 antibody
    • MAT antibody
    • mitochondrial acetoacetyl-CoA thiolase antibody
    • mitochondrial antibody
    • RATACAL antibody
    • T2 antibody
    • testicular tissue protein Li 198 antibody
    • THIL antibody
    • THIL_HUMAN antibody
    see all

Anti-ACAT1 antibody [9H10AB4] 画像

  • Immunocytochemistry image of ab110290 stained human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min). The cells were incubated with the antibody (9H10AB4, 5 µg/ml) for 2 hours at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria.
  • ab110290 immunocaptured from HepG2 cells (lane 1) and Human liver mitochondria (lane 2)

    Predicted molecular weight is 44 kDa.
  • ab110290, at 1 µg/mL, staining ACAT1 in HeLa (blue) or in an isotype control antibody (red) and analyzed by flow cytometry.
  • ACAT1 immunohistochemistry in human cerebellum visualized with ab110290. ACAT1 immunoactivity is most intense in neuronal cell bodies, most notably in the large Purkinje cells. Note the distinctive subcellular localization of ACAT1 immunoreactivity in the Purkinje cell bodies. The functional significance of this pattern is unknown at present but this antibody offers the opportunity to investigate it in more detail.

Anti-ACAT1 antibody [9H10AB4] (ab110290) 使用論文

ab110290 has not yet been referenced specifically in any publications.

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