Anti-ACADVL/VLCAD 抗体 [6A9AF2] (ab110285)
Key features and details
- Mouse monoclonal [6A9AF2] to ACADVL/VLCAD
- Suitable for: ICC/IF, IP, IHC-P
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-ACADVL/VLCAD antibody [6A9AF2]
ACADVL/VLCAD 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [6A9AF2] to ACADVL/VLCAD -
由来種
Mouse -
アプリケーション
適用あり: ICC/IF, IP, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Tissue, cells or virus. This information is considered to be commercially sensitive.
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ポジティブ・コントロール
- Human HDFn cells; Human liver mitochondria; Human cerebellum tissue.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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精製度
Proprietary Purification -
特記事項(精製)
The antibody was produced in vitro using hybridoma grown in serum-free media and then purified by biochemical fractionation. Purity >95% by SDS-PAGE. -
ポリ/モノ
モノクローナル -
クローン名
6A9AF2 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab110285の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 5 µg/ml.
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IP |
Use at an assay dependent concentration.
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IHC-P |
1/250. Perform heat mediated antigen retrieval - 1 min pressure cook in 1mmol EDTA pH8.
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特記事項 |
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ICC/IF
Use a concentration of 5 µg/ml. |
IP
Use at an assay dependent concentration. |
IHC-P
1/250. Perform heat mediated antigen retrieval - 1 min pressure cook in 1mmol EDTA pH8. |
ターゲット情報
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機能
Active toward esters of long-chain and very long chain fatty acids such as palmitoyl-CoA, mysritoyl-CoA and stearoyl-CoA. Can accomodate substrate acyl chain lengths as long as 24 carbons, but shows little activity for substrates of less than 12 carbons. -
パスウェイ
Lipid metabolism; mitochondrial fatty acid beta-oxidation. -
関連疾患
Defects in ACADVL are the cause of acyl-CoA dehydrogenase very long chain deficiency (ACADVLD) [MIM:201475]. ACADVLD is an autosomal recessive disease which leads to impaired long-chain fatty acid beta-oxidation. It is clinically heterogeneous, with three major phenotypes: a severe childhood form, with early onset, high mortality, and high incidence of cardiomyopathy; a milder childhood form, with later onset, usually with hypoketotic hypoglycemia as the main presenting feature, low mortality, and rare cardiomyopathy; and an adult form, with isolated skeletal muscle involvement, rhabdomyolysis, and myoglobinuria, usually triggered by exercise or fasting. -
配列類似性
Belongs to the acyl-CoA dehydrogenase family. -
細胞内局在
Mitochondrion inner membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 37 Human
- Omim: 609575 Human
- SwissProt: P49748 Human
- Unigene: 437178 Human
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別名
- ACAD 6 antibody
- ACAD6 antibody
- ACADV_HUMAN antibody
see all
画像
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Immunocytochemistry image of ACADVL/VLCAD antibody [6A9AF2] (ab110285) stained human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated with ab110285 at 5 µg/ml for 2h at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria.
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ab110285,at 1/250 dilution, staining ACADVL/VLCAD in formalin-fixed, paraffin-embedded human cerebellum tissue. Immunoactivity is most intense in neuronal cell bodies, most notably in the large Purkinje cells.
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ACADVL/VLCAD immunocaptured from 0.75mg of human liver mitochondria lysate /10ul antibody conjugated beads using ab110285.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab110285 は 1 報の論文で使用されています。
- Sweeney TE et al. A toll-like receptor 2 pathway regulates the Ppargc1a/b metabolic co-activators in mice with Staphylococcal aureus sepsis. PLoS One 6:e25249 (2011). WB ; Mouse . PubMed: 21966468