製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ACADS
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rat, Horse, Chicken, Cow, Pig, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human ACADS.

    (Peptide available as ab91440.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following human tissue lysates: liver; heart; skeletal muscle.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab79169 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • パスウェイ
    Lipid metabolism; mitochondrial fatty acid beta-oxidation.
  • 関連疾患
    Defects in ACADS are the cause of acyl-CoA dehydrogenase short-chain deficiency (ACADSD) [MIM:201470]. It is an autosomal recessive disorder resulting in acute acidosis and muscle weakness in infants, and a form of lipid-storage myopathy in adults.
  • 配列類似性
    Belongs to the acyl-CoA dehydrogenase family.
  • 細胞内局在
    Mitochondrion matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ACAD3 antibody
    • ACADS antibody
    • ACADS_HUMAN antibody
    • Acyl Coenzyme A dehydrogenase, C2 to C3 short chain antibody
    • Acyl-CoA dehydrogenase, C2 to C3 short chain antibody
    • Acyl-CoA dehydrogenase, short chain antibody
    • Acyl-Coenzyme A dehydrogenase, short chain antibody
    • AI196007 antibody
    • Bcd-1 antibody
    • Bcd1 antibody
    • Butyryl CoA dehydrogenase antibody
    • Butyryl-CoA dehydrogenase antibody
    • EC 1.3.99.2 antibody
    • mitochondrial antibody
    • SCAD antibody
    • Short chain acyl CoA dehydrogenase antibody
    • Short-chain specific acyl-CoA dehydrogenase antibody
    • Short-chain specific acyl-CoA dehydrogenase, mitochondrial antibody
    • Unsaturated acyl CoA reductase antibody
    see all

画像

  • All lanes : Anti-ACADS antibody (ab79169) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Human heart tissue lysate - total protein (ab29431)
    Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 44 kDa
    Observed band size : 44 kDa
    Additional bands at : 68 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes
  • ICC/IF image of ab79169 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79169, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) Hek293, HepG2 and MCF7 cells at 5µg/ml, and in 4% PFA fixed (10 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.

参考文献

ab79169 has not yet been referenced specifically in any publications.

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