製品の概要
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製品名Anti-ACADS antibody
ACADS 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to ACADS
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由来種Rabbit
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アプリケーション適用あり: WB, ICC/IFmore details
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種交差性交差種: Human
交差が予測される動物種: Rat, Horse, Chicken, Cow, Pig, Orangutan
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human ACADS.
(Peptide available as ab91440.)
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ポジティブ･コントロール
- This antibody gave a positive signal in the following human tissue lysates: liver; heart; skeletal muscle.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab79169 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa). | |
| ICC/IF | Use a concentration of 5 µg/ml. |
ターゲット情報
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パスウェイLipid metabolism; mitochondrial fatty acid beta-oxidation.
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関連疾患Defects in ACADS are the cause of acyl-CoA dehydrogenase short-chain deficiency (ACADSD) [MIM:201470]. It is an autosomal recessive disorder resulting in acute acidosis and muscle weakness in infants, and a form of lipid-storage myopathy in adults.
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配列類似性Belongs to the acyl-CoA dehydrogenase family.
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細胞内局在Mitochondrion matrix.
- Information by UniProt
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参照データベース
- Entrez Gene: 35 Human
- Entrez Gene: 64304 Rat
- Omim: 606885 Human
- SwissProt: P16219 Human
- SwissProt: P15651 Rat
- Unigene: 507076 Human
- Unigene: 1167 Rat
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別名
- ACAD3 antibody
- ACADS antibody
- ACADS_HUMAN antibody
see all
画像
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Western blot - ACADS antibody (ab79169)All lanes : Anti-ACADS antibody (ab79169) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : Human heart tissue lysate - total protein (ab29431)
Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 44 kDa
Observed band size: 44 kDa
Additional bands at: 68 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes -
Immunocytochemistry/ Immunofluorescence - ACADS antibody (ab79169)ICC/IF image of ab79169 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79169, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) Hek293, HepG2 and MCF7 cells at 5µg/ml, and in 4% PFA fixed (10 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.
参考文献
ab79169 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"