製品の概要

  • 製品名Anti-ACADS antibody [7E1AB5]
    ACADS 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [7E1AB5] to ACADS
  • アプリケーション適用あり: WB, ICC/IF, In-Cell ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length protein Human ACADS

  • ポジティブ・コントロール
    • Fibroblast, HeLa, HepG2. Human Heart FFPE tissue.
  • 特記事項

    Product was previously marketed under the MitoSciences sub-brand.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab110318 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 44 kDa.
ICC/IF Use a concentration of 1 µg/ml.
In-Cell ELISA Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 5 µg/ml.

ターゲット情報

  • パスウェイLipid metabolism; mitochondrial fatty acid beta-oxidation.
  • 関連疾患Defects in ACADS are the cause of acyl-CoA dehydrogenase short-chain deficiency (ACADSD) [MIM:201470]. It is an autosomal recessive disorder resulting in acute acidosis and muscle weakness in infants, and a form of lipid-storage myopathy in adults.
  • 配列類似性Belongs to the acyl-CoA dehydrogenase family.
  • 細胞内局在Mitochondrion matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ACAD3 antibody
    • ACADS antibody
    • ACADS_HUMAN antibody
    • Acyl Coenzyme A dehydrogenase, C2 to C3 short chain antibody
    • Acyl-CoA dehydrogenase, C2 to C3 short chain antibody
    • Acyl-CoA dehydrogenase, short chain antibody
    • Acyl-Coenzyme A dehydrogenase, short chain antibody
    • AI196007 antibody
    • Bcd-1 antibody
    • Bcd1 antibody
    • Butyryl CoA dehydrogenase antibody
    • Butyryl-CoA dehydrogenase antibody
    • EC 1.3.99.2 antibody
    • mitochondrial antibody
    • SCAD antibody
    • Short chain acyl CoA dehydrogenase antibody
    • Short-chain specific acyl-CoA dehydrogenase antibody
    • Short-chain specific acyl-CoA dehydrogenase, mitochondrial antibody
    • Unsaturated acyl CoA reductase antibody
    see all

Anti-ACADS antibody [7E1AB5] 画像

  • All lanes : Anti-ACADS antibody [7E1AB5] (ab110318) at 1 µg/ml

    Lane 1 : HepG2 whole cell cells at 10 µg
    Lane 2 : ACADS at 0.01 µg


    Predicted band size : 44 kDa
  • Immunocytochemistry image of stained human fibroblasts. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with the ab110318 (1 µg/ml) for 2 hours at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. The target protein locates to the mitochondrial matrix.
  • IHC image of ACADS staining in Human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110318, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-ACADS antibody [7E1AB5] (ab110318) 使用論文

ab110318 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"