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Human liver mitochondria
Product was previously marketed under the MitoSciences sub-brand.
Our Abpromise guarantee covers the use of ab110289 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use a concentration of 5 µg/ml. Heat-induced antigen retrieval improves signal|
|Flow Cyt||Use a concentration of 1 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|IP||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 44 kDa.|
|In-Cell ELISA||Use a concentration of 1 µg/ml.|
ACAA1 was immunoprecipitated using 0.5mg Mouse Liver tissue lysate, 5µg of Mouse monoclonal to ACAA1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Liver tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab110289.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.
Band: 44kDa; ACAA1