Rabbit polyclonal to ABLIM1
Rabbit, Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Orangutan
Synthetic peptide, corresponding to a region between residue 50 and 100 of human ABLIM1 (NP_002304.3)
HeLa and 293T whole cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate, pH 7-8
Concentration information loading...
Immunogen affinity purified
ab85808 was affinity purified using an epitope specific to ABLIM1 immobilized on solid support.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa).
Use at 5-10 µg/mg of lysate.
May act as scaffold protein (By similarity). May play a role in the development of the retina. Has been suggested to play a role in axon guidance.
Detected in liver, heart, skeletal muscle, brain and retina, where it is concentrated in the inner segment and in the outer plexiform layers.
Contains 1 HP (headpiece) domain.
Contains 4 LIM zinc-binding domains.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cytoplasm. Cytoplasm > cytoskeleton. Associated with the cytoskeleton.
Information by UniProt
ABLIM 1 antibody
Western blot - Anti-ABLIM1 antibody (ab85808)
All lanes : Anti-ABLIM1 antibody (ab85808) at 0.04 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Predicted band size: 88 kDa Observed band size: 88 kDa Exposure time: 3 minutes
Immunoprecipitation - Anti-ABLIM1 antibody (ab85808)
Detection of ABLIM1 by Western Blot of Immunprecipitate.
ab85808 at 1µg/ml staining ABLIM1 in HeLa whole cell lysate immunoprecipitated using ab85808 at 10µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.
has not yet been referenced specifically in any publications.
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