製品の概要

  • 製品名Anti-ABL2 antibody
    ABL2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to ABL2
  • アプリケーション適用あり: WB, ELISA, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Horse, Cow, Dog, Chimpanzee, Rhesus monkey
  • 免疫原

    Partial recombinant fragment corresponding to the internal amino acids 743-842 (KKTLGLRAGK PTASDDTSKP FPRSNSTSSM SSGLPEQDRM AMTLPRNCQR SKLQLERTVS TSSQPEENVD RANDMLPKKS EESAAPSRER PKAKLLPRGA) of human ABL2 (AAH65912) with a proprietary tag.

  • ポジティブ・コントロール
    • K-562 cell lysate. Human cerebellum tissue and HeLa cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab87987 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 128 kDa.
ELISA Use at an assay dependent dilution.
IHC-P Use a concentration of 1.5 µg/ml. Antigen retrieval is not essential but may optimise staining.
ICC/IF Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能Non-receptor tyrosine-protein kinase that plays an ABL1-overlapping role in key processes linked to cell growth and survival such as cytoskeleton remodeling in response to extracellular stimuli, cell motility and adhesion and receptor endocytosis. Coordinates actin remodeling through tyrosine phosphorylation of proteins controlling cytoskeleton dynamics like MYH10 (involved in movement); CTTN (involved in signaling); or TUBA1 and TUBB (microtubule subunits). Binds directly F-actin and regulates actin cytoskeletal structure through its F-actin-bundling activity. Involved in the regulation of cell adhesion and motility through phosphorylation of key regulators of these processes such as CRK, CRKL, DOK1 or ARHGAP35. Adhesion-dependent phosphorylation of ARHGAP35 promotes its association with RASA1, resulting in recruitment of ARHGAP35 to the cell periphery where it inhibits RHO. Phosphorylates multiple receptor tyrosine kinases like PDGFRB and other substrates which are involved in endocytosis regulation such as RIN1. In brain, may regulate neurotransmission by phosphorylating proteins at the synapse. ABL2 acts also as a regulator of multiple pathological signaling cascades during infection. Pathogens can highjack ABL2 kinase signaling to reorganize the host actin cytoskeleton for multiple purposes, like facilitating intracellular movement and host cell exit. Finally, functions as its own regulator through autocatalytic activity as well as through phosphorylation of its inhibitor, ABI1.
  • 組織特異性Widely expressed.
  • 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. ABL subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • ドメインContains two distinct classes of F-actin-binding domains. Although both can bind F-actin, the 2 are required to bundle actin filaments.
  • 翻訳後修飾Phosphorylated at Tyr-261 by ABL1 in response to oxidative stress. Phosphorylated by PDGFRB.
    Polyubiquitinated. Polyubiquitination of ABL2 leads to degradation.
  • 細胞内局在Cytoplasm, cytoskeleton.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Abelson murine leukemia viral oncogene homolog 2 antibody
    • Abelson related gene protein antibody
    • Abelson tyrosine-protein kinase 2 antibody
    • Abelson-related gene protein antibody
    • ABL2 antibody
    • ABL2_HUMAN antibody
    • ABLL antibody
    • ARG antibody
    • Tyrosine kinase ARG antibody
    • Tyrosine protein kinase ABL2 antibody
    • Tyrosine-protein kinase ARG antibody
    • v abl Abelson murine leukemia viral oncogene homolog 2 antibody
    see all

Anti-ABL2 antibody 画像

  • Anti-ABL2 antibody (ab87987) at 1 µg/ml + immunogen at 0.1 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
    Developed using the ECL technique

    Predicted band size : 128 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Anti-ABL2 antibody (ab87987) at 1 µg/ml + K562 cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 128 kDa
    Observed band size : 128 kDa
  • ab87987, at 1.5 µg/ml, detecting ABL2 in formalin fixed, paraffin embedded sections of human cerebellum tissue by immunohistochemistry.
  • ab87987, at 10 µg/ml, detecting ABL2 in HeLa cells by immunofluorescence.
  • ab87987, detecting ABL2 by sandwich ELISA. The detection limit is approximately 0.1 ng/ml when using ab87987 as a capture antibody.

Anti-ABL2 antibody (ab87987) 使用論文

ab87987 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (HCT116)
Gel Running Conditions Reduced Denaturing (4-20%)
Loading amount 50 µg
Specification HCT116
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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投稿 Nov 30 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"