製品の概要

  • 製品名Anti-ABI2 antibody [EPR3906]
    ABI2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR3906] to ABI2
  • アプリケーション適用あり: WB, IP, IHC-P, ICCmore details
    適用なし: Flow Cyt
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to a region within in Human ABI2.

  • ポジティブ・コントロール
    • HeLa, Jurkat, K562, SH-SY5Y and HepG2 cell lysates. Paraffin-embedded Human brain tissue.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 精製度Tissue culture supernatant
  • ポリ/モノモノクローナル
  • クローン名EPR3906
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab108340 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 57 kDa.
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/500 - 1/1000.
  • 追加情報Is unsuitable for Flow Cyt.
  • ターゲット情報

    • 機能May act in regulation of cell growth and transformation by interacting with nonreceptor tyrosine kinases ABL1 and/or ABL2. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Regulates ABL1/c-Abl-mediated phosphorylation of MENA.
    • 配列類似性Belongs to the ABI family.
      Contains 1 SH3 domain.
      Contains 1 t-SNARE coiled-coil homology domain.
    • 翻訳後修飾Is a substrate for ABL1.
    • 細胞内局在Cytoplasm and Cell projection > lamellipodium. Cell projection > filopodium. Cytoplasm > cytoskeleton. Isoform 1 but not isoform 3 is localized to protruding lamellipodia and filopodia tips.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Abelson interactor 2 antibody
      • ABI 2 antibody
      • Abi-2 antibody
      • ABI2 antibody
      • ABI2_HUMAN antibody
      • ABI2B antibody
      • Abl binding protein 3 antibody
      • Abl interacting protein 1 (SH3 containing protein) antibody
      • Abl interactor 2 antibody
      • Abl interactor protein 2b antibody
      • Abl-binding protein 3 antibody
      • AblBP3 antibody
      • AIP 1 antibody
      • Arg binding protein 1 antibody
      • Arg protein tyrosine kinase binding protein antibody
      • Arg-binding protein 1 antibody
      • ArgBP1 antibody
      • ARGBPIA antibody
      • ArgBPIB antibody
      • OTTHUMP00000163783 antibody
      • OTTHUMP00000163784 antibody
      • OTTHUMP00000206182 antibody
      • OTTHUMP00000206183 antibody
      • OTTHUMP00000206184 antibody
      • OTTHUMP00000206185 antibody
      • SSH3BP2 antibody
      see all

    Anti-ABI2 antibody [EPR3906] 画像



    • Performed under reducing conditions.

      Predicted band size : 57 kDa
      Observed band size : 80 kDa (why is the actual band size different from the predicted?)


      Exposure time : 2 minutes

      This image is courtesy of an anonymous Abreview

      Western blot analysis of Human fibroblast cell lysates (15μg/lane) labelling ABI2 with ab108340 at 1:1000. A Dyomics® 647-conjugated Goat anti-rabbit IgG monoclonal (1:5000) was used as the secondary antibody.

      See Abreview

    • ab108340, at 1/250, staining ABI2 in paraffin-embedded Human brain tissue by Immunohistochemistry.
    • ab108340, at 1/500, staining ABI2 in HeLa cells by Immunofluorescence.

    Anti-ABI2 antibody [EPR3906] (ab108340) 使用論文

    This product has been referenced in:
    • Crawford LJ  et al. Synergistic effects of proteasome inhibitor carfilzomib in combination with tyrosine kinase inhibitors in imatinib-sensitive and -resistant chronic myeloid leukemia models. Oncogenesis 3:e90 (2014). WB ; Human . Read more (PubMed: 24590311) »

    See 1 Publication for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Loading amount 15 µg
    Gel Running Conditions Reduced Denaturing (10%)
    Sample Human Cell lysate - whole cell (Fibroblasts)
    Specification Fibroblasts
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    投稿 Aug 14 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunoprecipitation
    Immuno-precipitation step Other - Dynabeads protein A
    Sample Human Cell lysate - whole cell (HEK 293T Cells)
    Specification HEK 293T Cells
    Total protein in input 600 µg
    Username

    Abcam user community

    Verified customer

    投稿 Aug 13 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"