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C-SEKSRYSLHQNMDK, corresponding to C terminal amino acids 252 - 265 of Human ABHD6 (NP_065727.3), with a cysteine residue linker Peptide available as ab95134.
Our Abpromise guarantee covers the use of ab87532 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC image of ABHD6 staining in Human Hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab87532, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
ab87532 has not yet been referenced specifically in any publications.