Anti-Abhd5/CGI-58 抗体 (ab59488)
Key features and details
- Goat polyclonal to Abhd5/CGI-58
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Abhd5/CGI-58 antibody
Abhd5/CGI-58 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Abhd5/CGI-58 -
由来種
Goat -
特異性
This antibody is not expected to cross-react with ABHD4. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Sheep, Dog, Pig, Orangutan -
免疫原
Synthetic peptide corresponding to Human Abhd5/CGI-58 aa 150-250 (internal sequence).
Database link: Q8WTS1 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab59488の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.2 - 0.6 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa).
A 1 hour primary incubation is recommended for this product. Additional faint bands of 26kDa and 18kDa were observed in NIH-3T3. These bands were successfully blocked by incubation with the immunizing peptide. |
特記事項 |
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WB
Use a concentration of 0.2 - 0.6 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa). A 1 hour primary incubation is recommended for this product. Additional faint bands of 26kDa and 18kDa were observed in NIH-3T3. These bands were successfully blocked by incubation with the immunizing peptide. |
ターゲット情報
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機能
Lysophosphatidic acid acyltransferase which functions in phosphatidic acid biosynthesis. May regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2. Involved in keratinocyte differentiation. -
組織特異性
Widely expressed in various tissues, including lymphocytes, liver, skeletal muscle and brain. Expressed by upper epidermal layers and dermal fibroblasts in skin, hepatocytes and neurons (at protein level). -
関連疾患
Defects in ABHD5 are the cause of Chanarin-Dorfman syndrome (CDS) [MIM:275630]; also called triglyceride storage disease with impaired long-chain fatty acid oxidation or neutral lipid storage disease with ichthyosis. CDS is an autosomal recessive inborn error of lipid metabolism with multisystemic accumulation of triglycerides although plasma concentrations are normal. Clinical characteristics are congenital generalized ichthyosis, vacuolated leukocytes, hepatomegaly, myopathy, cataracts, neurosensory hearing loss and developmental delay. The disorder presents at birth with generalized, fine, white scaling of the skin and a variable degree of erythema resembling non-bullous congenital ichthyosiform erythroderma. -
配列類似性
Belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily. -
発生段階
Detected in fetal epidermis from 49 to 135 days estimated gestational age (at protein level). -
ドメイン
The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate. -
細胞内局在
Cytoplasm. Lipid droplet. Colocalized with PLIN and ADRP on the surface of lipid droplets. The localization is dependent upon the metabolic status of the adipocytes and the activity of PKA. - Information by UniProt
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参照データベース
- Entrez Gene: 485570 Dog
- Entrez Gene: 51099 Human
- Entrez Gene: 67469 Mouse
- Entrez Gene: 497624 Pig
- Entrez Gene: 316122 Rat
- Entrez Gene: 100125355 Sheep
- Omim: 604780 Human
- SwissProt: Q8WTS1 Human
see all -
別名
- 1-acylglycerol-3-phosphate O-acyltransferase ABHD5 antibody
- ABHD5 antibody
- ABHD5_HUMAN antibody
see all
画像
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Anti-Abhd5/CGI-58 antibody (ab59488) at 1 µg/ml + A431 cell line lysate (35µg protein in RIPA buffer)
Predicted band size: 39 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence. -
Anti-Abhd5/CGI-58 antibody (ab59488) at 0.2 µg/ml + NIH-3T3 cell lysate (RIPA buffer) at 35 µg
Predicted band size: 39 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab59488 は 4 報の論文で使用されています。
- Erb SJ et al. Responsiveness of PNPLA3 and lipid-related transcription factors is dependent upon fatty acid profile in primary bovine hepatocytes. Sci Rep 12:888 (2022). PubMed: 35042927
- Lim S et al. Ginsenoside Rb1 Induces Beta 3 Adrenergic Receptor-Dependent Lipolysis and Thermogenesis in 3T3-L1 Adipocytes and db/db Mice. Front Pharmacol 10:1154 (2019). PubMed: 31680950
- Jebessa ZH et al. The lipid droplet-associated protein ABHD5 protects the heart through proteolysis of HDAC4. Nat Metab 1:1157-1167 (2019). PubMed: 31742248
- Lin YY et al. KSRP and MicroRNA 145 Are Negative Regulators of Lipolysis in White Adipose Tissue. Mol Cell Biol 34:2339-2349 (2014). PubMed: 24732799