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RabMAb

Anti-ABCG1 抗体 [EP1366Y] (ab52617)

製品の概要

  • 製品名
    Anti-ABCG1 antibody [EP1366Y]
    ABCG1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1366Y] to ABCG1
  • アプリケーション
    適用あり: ICC/IF, WBmore details
    適用なし: IHC-P
  • 種交差性
    交差種: Mouse, Rat, Cow, Human
  • 免疫原

    A synthetic peptide corresponding to residues on human ABCG1.

  • ポジティブ・コントロール
    • HUVEC cell lysate, human cervical carcinoma.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

アプリケーション

Our Abpromise guarantee covers the use of ab52617 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/100.
WB 1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 75 kDa).
  • 追加情報
    Is unsuitable for IHC-P.
  • ターゲット情報

    • 機能
      Transporter involved in macrophage lipid homeostasis. Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages.
    • 組織特異性
      Expressed in several tissues. Expressed in macrophages; expression is increased in macrophages from patients with Tangier disease.
    • 配列類似性
      Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
      Contains 1 ABC transmembrane type-2 domain.
      Contains 1 ABC transporter domain.
    • 細胞内局在
      Endoplasmic reticulum membrane. Golgi apparatus membrane. Predominantly localized in the intracellular compartments mainly associated with the endoplasmic reticulum (ER) and Golgi membranes.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ABC transporter 8 antibody
      • ABC8 antibody
      • ABCG1 antibody
      • ABCG1_HUMAN antibody
      • ATP-binding cassette sub family G member 1 antibody
      • ATP-binding cassette sub-family G member 1 antibody
      • ATP-binding cassette transporter 8 antibody
      • ATP-binding cassette transporter member 1 of subfamily G antibody
      • ATP-binding cassette, sub family G WHITE member 1 antibody
      • homolog of Drosophila white antibody
      • MGC34313 antibody
      • White protein homolog antibody
      • White protein homolog ATP binding cassette transporter 8 antibody
      • WHITE1 antibody
      • WHT1 antibody
      see all

    Anti-ABCG1 antibody [EP1366Y] 画像

    • All lanes : Anti-ABCG1 antibody [EP1366Y] (ab52617) at 20 µg

      Lane 1 : Rat kidney lysate
      Lane 2 : Rat liver lysate
      Lane 3 : MDBK (Bovine kidney cell line) whole cell lysate
      Lane 4 : HUVEC (Human umbilical vein endothelial cell line) whole cell lysate
      Lane 5 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
      Lane 6 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 20000 µg

      Predicted band size : 75 kDa

      Blocking and diluting buffer: 5% NFDM /TBST

    • ab52617 staining ABCG1 in HUVEC (Human umbilical vein endothelial cell line) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. ab195889 was used as a counterstain for primary antibody ab52617 at 1/200. DAPI was used as a nuclear counterstain and PBS as a negative control.

    • Anti-ABCG1 antibody [EP1366Y] (ab52617) at 1/2000 dilution + HUVEC cell lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP antiibody at 1/2000 dilution

      Predicted band size : 75 kDa
      Observed band size : 110 kDa (why is the actual band size different from the predicted?)
    • Ab52617 at 1/50 dilution staining human cervical carcinoma tissue; paraffin embedded.

    Anti-ABCG1 antibody [EP1366Y] (ab52617) 使用論文

    This product has been referenced in:
    • Na TY  et al. The trisaccharide raffinose modulates epidermal differentiation through activation of liver X receptor. Sci Rep 7:43823 (2017). WB ; Human . Read more (PubMed: 28266648) »
    • Cheng-Mao X  et al. Placental ABCA1 Expression Is Increased in Spontaneous Preterm Deliveries Compared with Iatrogenic Preterm Deliveries and Term Deliveries. Biomed Res Int 2017:8248094 (2017). WB, IHC, RT-PCR ; Human . Read more (PubMed: 28630870) »

    See all 16 Publications for this product

    Product Wall

    Application
    Western blot
    Sample
    Rabbit Tissue lysate - whole (Synovial membrane)
    Gel Running Conditions
    Reduced Denaturing (8%)
    Loading amount
    35 µg
    Specification
    Synovial membrane
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Jun 13 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Antigen retrieval step
    None
    Permeabilization
    Yes - 0.1M PBST with 10%donkeys serum
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Fixative
    Paraformaldehyde
    Username

    Dr. Ruma Raha-Chowdhury

    Verified customer

    投稿 Feb 12 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Brain lysate, Liver lysate and human serum)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    Brain lysate, Liver lysate and human serum
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Username

    Dr. Ruma Raha-Chowdhury

    Verified customer

    投稿 Jan 28 2016

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HepG2)
    Specification
    HepG2
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Protein Block (serum free) as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    投稿 Oct 13 2008

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Cow Cell lysate - whole cell (endothelial cells from cerebral capillaries)
    Loading amount
    20 µg
    Specification
    endothelial cells from cerebral capillaries
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Username

    Dr. GOSSELET Fabien

    Verified customer

    投稿 Mar 20 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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