製品の概要

  • 製品名Anti-ABCG1 antibody
    ABCG1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ABCG1
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide, corresponding to internal sequence amino acids 300-400 of Human ABCG1 (Peptide available as ab97573.)

  • ポジティブ・コントロール
    • Mouse peritoneal macrophages.

アプリケーション

Our Abpromise guarantee covers the use of ab36969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
WB 1/2500. Detects a band of approximately 90 kDa (predicted molecular weight: 76 kDa).

ターゲット情報

  • 機能Transporter involved in macrophage lipid homeostasis. Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages.
  • 組織特異性Expressed in several tissues. Expressed in macrophages; expression is increased in macrophages from patients with Tangier disease.
  • 配列類似性Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
    Contains 1 ABC transmembrane type-2 domain.
    Contains 1 ABC transporter domain.
  • 細胞内局在Endoplasmic reticulum membrane. Golgi apparatus membrane. Predominantly localized in the intracellular compartments mainly associated with the endoplasmic reticulum (ER) and Golgi membranes.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ABC transporter 8 antibody
    • ABC8 antibody
    • ABCG1 antibody
    • ABCG1_HUMAN antibody
    • ATP-binding cassette sub family G member 1 antibody
    • ATP-binding cassette sub-family G member 1 antibody
    • ATP-binding cassette transporter 8 antibody
    • ATP-binding cassette transporter member 1 of subfamily G antibody
    • ATP-binding cassette, sub family G WHITE member 1 antibody
    • homolog of Drosophila white antibody
    • MGC34313 antibody
    • White protein homolog antibody
    • White protein homolog ATP binding cassette transporter 8 antibody
    • WHITE1 antibody
    • WHT1 antibody
    see all

Anti-ABCG1 antibody 画像

  • All lanes : Anti-ABCG1 antibody (ab36969)

    Lane 1 : Positive ABCG1 control detected in mouse peritoneal macrophages (40 µg)
    Lane 2 : 10 µM 22-(R)-hydocholesterol treated mouse peritoneal macrophages (40 µg)
    Lane 3 : 100 nM TO901713 (LXRa ligand) treated mouse peritoneal macrophages (40 µg)
    Lane 4 : 500 nM TO91713 (LXRa ligand) treated mouse peritoneal macrophages (40 µg)

    Developed using the ECL technique

    Predicted band size : 76 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 60 kDa (possible non-specific binding).
  • ab36969 staining ABCG1 in lung and alveolar macrophages by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Anti-ABCG1 antibody (ab36969) 使用論文

This product has been referenced in:
  • Voloshyna I  et al. Infliximab reverses suppression of cholesterol efflux proteins by TNF-a: a possible mechanism for modulation of atherogenesis. Biomed Res Int 2014:312647 (2014). Human . Read more (PubMed: 24587984) »
  • Zhao J  et al. Retinoic acid isomers facilitate apolipoprotein E production and lipidation in astrocytes through the retinoid X receptor/retinoic acid receptor pathway. J Biol Chem 289:11282-92 (2014). WB ; Human . Read more (PubMed: 24599963) »

See all 8 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Sample Mouse Cell (Macrophages)
Specification Macrophages
Permeabilization Yes - PBS+0.05% saponin
Fixative Formaldehyde
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投稿 Mar 30 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (macrophages)
Loading amount 30 µg
Specification macrophages
Treatment acLDL 24h
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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投稿 Oct 02 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (THP1, HEK293)
Loading amount 15 µg
Specification THP1, HEK293
Treatment 1uM TO-1317
Gel Running Conditions Reduced Denaturing
Blocking step 5%milk+1%BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C
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投稿 Jan 26 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"