製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ABCB8
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within the internal sequence amino acids 540-589 (EPVLFGTTIM ENIRFGKLEA SDEEVYTAAR EANAHEFITS FPEGYNTVVG) of Human ABCB8 (NP_009119)

  • ポジティブ・コントロール
    • Fetal heart lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab83194 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 80 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay 1/62500.

ターゲット情報

  • 組織特異性
    Ubiquitous.
  • 配列類似性
    Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
    Contains 1 ABC transmembrane type-1 domain.
    Contains 1 ABC transporter domain.
  • 細胞内局在
    Mitochondrion inner membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ATP binding cassette sub family B member 8 antibody
    • ABCB8 antibody
    • ABCB8_HUMAN antibody
    • ATP binding cassette sub family B (MDR/TAP) member 8 antibody
    • ATP binding cassette sub family B member 8 mitochondrial antibody
    • ATP-binding cassette sub-family B member 8 antibody
    • EST328128 antibody
    • M ABC1 antibody
    • M-ABC1 antibody
    • MABC1 antibody
    • Mitochondrial ABC protein antibody
    • mitochondrial antibody
    • Mitochondrial ATP binding cassette 1 antibody
    • Mitochondrial ATP-binding cassette 1 antibody
    see all

画像

  • Anti-ABCB8 antibody (ab83194) at 1 µg/ml + Human fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 80 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

ab83194 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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