製品の概要

  • 製品名Anti-ABCB4 antibody
    ABCB4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ABCB4
  • アプリケーション適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    A KLH conjugated synthetic peptide selected selected from the central region of human ABCB4.

  • ポジティブ・コントロール
    • Human hepatocarcinoma tissue and mouse heart tissue lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab71792 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
IHC-P
  • 追加情報ELISA: 1/1000.
    IHC-P: 1/50 - 1/100.
    WB: 1/100 - 1/500. Detects bands of approximately 40, 43, 47, 75 and 170 kDa (predicted molecular weight: 141 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Mediates ATP-dependent export of organic anions and drugs from the cytoplasm. Hydrolyzes ATP with low efficiency. Human MDR3 is not capable of conferring drug resistance. Mediates the translocation of phosphatidylcholine across the canalicular membrane of the hepatocyte.
    • 関連疾患Defects in ABCB4 are the cause of progressive familial intrahepatic cholestasis type 3 (PFIC3) [MIM:602347]. PFIC3 is an autosomal recessive liver disorder presenting with early onset cholestasis that progresses to cirrhosis and liver failure before adulthood. It is characterized by elevated serum gamma-glutamyltransferase levels.
      Defects in ABCB4 are a cause of intrahepatic cholestasis of pregnancy (ICP) [MIM:147480]; also known as obstetric cholestasis. ICP is a multifactorial liver disorder of pregnancy. It presents during the second or, more commonly, the third trimestre of pregnancy with intense pruritus which becomes more severe with advancing gestation and cholestasis. Cholestasis results from abnormal biliary transport from the liver into the small intestine. ICP causes fetal distress, spontaneous premature delivery and intrauterine death. ICP patients have spontaneous and progressive disappearance of cholestasis after delivery.
      Defects in ABCB4 are a cause of gallbladder disease type 1 (GBD1) [MIM:600803]. It is one of the major digestive diseases. Gallstones composed of cholesterol (cholelithiasis) are the common manifestations in western countries. Most people with gallstones, however, remain asymptomatic through their lifetimes.
    • 配列類似性Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
      Contains 2 ABC transmembrane type-1 domains.
      Contains 2 ABC transporter domains.
    • 細胞内局在Cell membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ABC 21 antibody
      • ABC B4 antibody
      • ABC21 antibody
      • ABCB 4 antibody
      • Abcb4 antibody
      • ABCB4 protein antibody
      • ATP binding cassette sub family B MDR/TAP member 4 antibody
      • ATP binding cassette sub family B member 4 antibody
      • ATP-binding cassette sub-family B member 4 antibody
      • MDR 3 antibody
      • MDR2 antibody
      • MDR2/3 antibody
      • MDR3 antibody
      • MDR3 P glycoprotein antibody
      • MDR3 P gp antibody
      • MDR3_HUMAN antibody
      • Multidrug resistance protein 3 antibody
      • Multiple drug resistance 3 antibody
      • P glycoprotein 3 antibody
      • P-glycoprotein 3 antibody
      • PFIC 3 antibody
      • PFIC3 antibody
      • PGY 3 antibody
      • PGY3 antibody
      see all

    Anti-ABCB4 antibody 画像

    • Anti-ABCB4 antibody (ab71792) at 1/100 dilution + Mouse heart tissue lysates at 12.5 µg

      Secondary
      HRP-anti-rabbit

      Predicted band size : 141 kDa
      Observed band size : 170 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 40& 43 kDa,47 kDa,75 kDa. We are unsure as to the identity of these extra bands.
    • Formalin-fixed, paraffin-embedded human hepatocarcinoma tissue reacted with ab71792 at a 1:50 dilution, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

    Anti-ABCB4 antibody (ab71792) 使用論文

    This product has been referenced in:
    • Martin GG  et al. Ablating L-FABP in SCP-2/SCP-x null mice impairs bile acid metabolism and biliary HDL-cholesterol secretion. Am J Physiol Gastrointest Liver Physiol 307:G1130-43 (2014). Read more (PubMed: 25277800) »

    See 1 Publication for this product

    Product Wall

    Thank you for contacting me with an update. I'm very sorry to hear the replacement has also not provided satisfactory results.

    I would like to reassure you that the replacement is also covered by the 6 month guarantee.

    Before proc...

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    Thank you for getting back to us with your progress with the anti-ABCB4 antibody (ab71792). I am sorry to hear this antibody is still not providing satisfactory results.
    Kate is currently not in the office but I would be happy to arrange for a fre...

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    Thank you for taking the time to complete our questionnaire. The details you have kindly provided will provide us with vital information for our monitoring of product quality.

    I appreciate the time you have spent in the laboratory and unders...

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    Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

    I would like to reassure you that this antibody is tested and covered by our 6 month guarantee for WB ...

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    After sending my reply yesterday about ab71792 and alternatives, I learned that the immunogen for ab71792 shares 79% identity with the rat ABCB4, 11/14 amino acids. This may be enough to predict cross-reactivity but we cannot be sure unless identity is...

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    Thank you for your interest in ab71792. Which application do you need the antibody for: western blotting, IHC, or other?
    None of the ABCB4 antibodies have been tested with rat tissue, but one or more may cross-react. I am a little concerned about ...

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    Thank you for contacting us. ab3364; anti P Glycoprotein is no longer available in our catalogue. However we have similar products available to buy. The catalogue numbers are ab3083; ab3366; ab10333; ab80594; ab80626. Please check the da...

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