Anti-ABCB11 抗体 - Carboxyterminal end (ab71793)

製品の概要

  • 製品名Anti-ABCB11 antibody - Carboxyterminal end
    ABCB11 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ABCB11 - Carboxyterminal end
  • 特異性We have received some negative reports from customers using this antibody in IHC, therefore we do not recommend this antibody for IHC.
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    A KLH conjugated synthetic peptide selected from the C-terminal region of human ABCB11.

  • ポジティブ・コントロール
    • Human hepatocarcinoma tissue and mouse liver tissue lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab71793 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/500. Detects a band of approximately 146 kDa (predicted molecular weight: 146 kDa).
ELISA 1/1000.

ターゲット情報

  • 機能Involved in the ATP-dependent secretion of bile salts into the canaliculus of hepatocytes.
  • 組織特異性Expressed predominantly, if not exclusively in the liver, where it was further localized to the canalicular microvilli and to subcanalicular vesicles of the hepatocytes by in situ.
  • 関連疾患Defects in ABCB11 are the cause of progressive familial intrahepatic cholestasis type 2 (PFIC2) [MIM:601847]. PFIC2 is an inherited liver disease of childhood which is characterized by cholestasis and normal serum gamma-glutamyltransferase activity. Defects in ABCB11 are also found in cases of chronic intrahepatic cholestasis without obvious familial history of chronic liver disease.
    Defects in ABCB11 are the cause of benign recurrent intrahepatic cholestasis type 2 (BRIC2) [MIM:605479]. BRIC is characterized by intermittent episodes of cholestasis without progression to liver failure. There is initial elevation of serum bile acids, followed by cholestatic jaundice which generally spontaneously resolves after periods of weeks to months. The cholestatic attacks vary in severity and duration and patients are asymptomatic between episodes, both clinically and biochemically.
  • 配列類似性Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
    Contains 2 ABC transmembrane type-1 domains.
    Contains 2 ABC transporter domains.
  • ドメインMultifunctional polypeptide with two homologous halves, each containing an hydrophobic membrane-anchoring domain and an ATP binding cassette (ABC) domain.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ABC member 16, MDR/TAP subfamily antibody
    • ABCB1 antibody
    • Abcb11 antibody
    • ABCBB_HUMAN antibody
    • ATP binding cassette sub family B (MDR/TAP) member 11 antibody
    • ATP binding cassette sub family B member 11 antibody
    • ATP-binding cassette sub-family B member 11 antibody
    • Bile salt export pump antibody
    • BRIC2 antibody
    • Bsep antibody
    • PGY4 antibody
    • progressive familial intrahepatic cholestasis 2 antibody
    • Sister of P glycoprotein antibody
    • Spgp antibody
    see all

Anti-ABCB11 antibody - Carboxyterminal end 画像

  • Anti-ABCB11 antibody - Carboxyterminal end (ab71793) at 1/100 dilution + mouse liver tissue lysate at 12.5 µg

    Predicted band size : 146 kDa
    Observed band size : 146 kDa

Anti-ABCB11 antibody - Carboxyterminal end (ab71793) 使用論文

ab71793 has not yet been referenced specifically in any publications.

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Thank you for contacting us. I am sorry to hear you are experiencing difficulties with one of our products and that you do not observe a staining in IHC. We take product complaints very seriously, and investigate every product that we feel may not be p...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Human hepatoma cells Huh7)
Loading amount 25 µg
Specification Human hepatoma cells Huh7
Treatment 0.5uM GW4064
Gel Running Conditions Reduced Denaturing (4-12% gradient gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Abcam user community

Verified customer

投稿 Oct 05 2011

Thank you for your enquiry. I have contacted the originator of the product for more information, unfortunately the exact immunogen sequence information is proprietary. However, they did released information that the sequence is a 15 amino acid prot...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"