Anti-ABCA12 抗体 (ab98976)


  • 製品名Anti-ABCA12 antibody
    ABCA12 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ABCA12
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 1979-2028 (TTIFKMLTGD IIPSSGNILI RNKTGSLGHV DSHSSLVGYC PQEDALDDLV) of Human ABCA12 (NP_056472; UniProt ID: Q86UK0 isoform 2).

  • ポジティブ・コントロール
    • Human fetal stomach lysate



Our Abpromise guarantee covers the use of ab98976 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 257 kDa.Can be blocked with ABCA12 peptide (ab127886). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


  • 機能Probable transporter involved in lipid homeostasis.
  • 組織特異性Mainly expressed in the stomach, placenta, testis and fetal brain.
  • 関連疾患Defects in ABCA12 are the cause of ichthyosis harlequin (HI) [MIM:242500]; also known as harlequin fetus. HI is a very severe skin disorder in which the neonate is born with a thick covering of armor-like scales. The skin dries out to form hard diamond-shaped plaques separated by fissures, resembling 'armor plating'. The normal facial features are severely affected, with distortion of the lips (eclabion), eyelids (ectropion), ears, and nostrils. Affected babies are often born prematurely and rarely survive the perinatal period.
    Defects in ABCA12 are the cause of ichthyosis lamellar type 2 (LI2) [MIM:601277]; also known as ichthyosis congenita IIB (ICR2B). LI is a non-bullous ichthyosis, a skin disorder characterized by abnormal cornification of the epidermis. It is one the most severe forms of ichthyoses apparent at birth and persisting throughout life. LI patients are born encased in a tight, shiny, translucent covering called collodion membrane. Over the first weeks of life, the collodion membrane is gradually replaced by generalized large, dark brown, plate-like scales with minimal to no erythroderma. Tautness of facial skin commonly results in ectropion, eclabium and scarring alopecia of the scalp. Common complications are severe heat intolerance and recurrent ear infections.
  • 配列類似性Belongs to the ABC transporter superfamily. ABCA family.
    Contains 2 ABC transporter domains.
  • ドメインMultifunctional polypeptide with two homologous halves, each containing an hydrophobic membrane-anchoring domain and an ATP binding cassette (ABC) domain.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ABC transporter A family member 12 antibody
    • ABC transporter ABCA.12 antibody
    • ABC12 antibody
    • ABCA12 antibody
    • ABCAC_HUMAN antibody
    • AtABCA12 antibody
    • ATH16 antibody
    • ATP binding cassette 12 antibody
    • ATP binding cassette sub family A (ABC1) member 12 antibody
    • ATP binding cassette sub family A member 12 antibody
    • ATP binding cassette transporter 12 antibody
    • ATP-binding cassette 12 antibody
    • ATP-binding cassette sub-family A member 12 antibody
    • ATP-binding cassette transporter 12 antibody
    • Ichthyosis congenita II lamellar ichthyosis B antibody
    • ICR2B antibody
    • LI2 antibody
    • Putative ABC2 homolog 16 antibody
    see all

Anti-ABCA12 antibody 画像

  • Anti-ABCA12 antibody (ab98976) at 1 µg/ml + Human fetal stomach lysate at 10 µg

    Predicted band size : 257 kDa

Anti-ABCA12 antibody (ab98976) 使用論文

ab98976 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (human primary fibroblast)
Loading amount 30 µg
Specification human primary fibroblast
Gel Running Conditions Reduced Denaturing (7% acrylamide gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Mr. Jian Yuan-Ren

Verified customer

投稿 Apr 12 2012

Thank you very much for your reply.

I understand the customer's frustration, however I can not offer a free of charge replacement in this case as the antibody was working as stated on the datasheet.

If the customercould submit an Ab...

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Thank you for this update!

I am very happy to hear that the suggestions seemed to have resolved the problem. Unfortunately, there was no image attached to the email. Can you please send it again?

Thank you very much!

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigate this...

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Thank you for your enquiry. I can confirm that as the datasheet indicates ab98976 has only been tested and characterized for Western blot application. Currently, we do not have any data regarding IHC, though it does not necessary mean that ab98976 i...

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Thank you very much for your kind reply and the image.

Indeed, the results look very good to me. If I understood correctly, the 3rd band is where the customer used the sample with the mutated ABCA12.

The second band all on top could...

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