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Recombinant fragment corresponding to Human ABCA1 aa 1800-2260.
Database link: O95477
Our Abpromise guarantee covers the use of ab18180 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||Use at an assay dependent concentration.|
|Flow Cyt||Use 1-2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|WB||1/200 - 1/500. Predicted molecular weight: 254 kDa.
Abcam recommends using BSA as the blocking agent and 80-100 ug of lysate.
|ELISA||Use at an assay dependent concentration.
Direct ELISA, where a 96-well plate was coated with recombinant ABCA1 C-terminal protein. The antibody was then added to the wells and a goat anti-mouse IgG HRP conjugate was used for colour development. For ABCA1 quantification, a pair of anti-ABCA1 antibodies should be used to formulate a Sandwich ELISA and with a presence of an ABCA1 protein Standard. Optimal dilution to be determined by the end user.
The lower bands are from the secondary anti-mouse antibody reacting to the endogenous mouse antibodies from the tissue.