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RabMAb

Anti-ABAT 抗体 [EPR4433] (ab108249)

製品の概要

  • 製品名
    Anti-ABAT antibody [EPR4433]
    ABAT 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4433] to ABAT
  • 由来種
    Rabbit
  • 特異性
    Mouse cross reactivity tested by western blot and IHC. Rat cross reactivity tested by western blot only.
  • アプリケーション
    適用あり: WB, IP, IHC-Pmore details
    適用なし: Flow Cyt or ICC/IF
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human ABAT aa 1-100. The exact sequence is proprietary.
    (Peptide available as ab202287)

  • ポジティブ・コントロール
    • HepG2 cell lysate, SH-SY5Y cell lysae, Human fetal liver tissue lysate, rat brain tissue lysate, rat kidney tissue lysate, mouse brain tissue lysate, Human liver tissue.
  • 特記事項

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108249 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000 - 1/50000. Predicted molecular weight: 56 kDa.
IP 1/10 - 1/100.
IHC-P 1/1000.

See IHC antigen retrieval protocols.

Antigen retrieval is recommended

For unpurified use at 1/250 - 1/500 dilution. 

  • 追加情報
    Is unsuitable for Flow Cyt or ICC/IF.
  • ターゲット情報

    • 機能
      Catalyzes the conversion of gamma-aminobutyrate and L-beta-aminoisobutyrate to succinate semialdehyde and methylmalonate semialdehyde, respectively. Can also convert delta-aminovalerate and beta-alanine.
    • 組織特異性
      Liver > pancreas > brain > kidney > heart > placenta.
    • 関連疾患
      Defects in ABAT are a cause of gamma-aminobutyrate transaminase deficiency (GABA-AT deficiency) [MIM:613163]. The phenotype of this deficiency includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities.
    • 配列類似性
      Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.
    • 細胞内局在
      Mitochondrion matrix.
    • Information by UniProt
    • 参照データベース
    • 別名
      • (S) 3 amino 2 methylpropionate transaminase antibody
      • (S)-3-amino-2-methylpropionate transaminase antibody
      • 4 aminobutyrate aminotransferase antibody
      • 4 aminobutyrate aminotransferase, mitochondrial antibody
      • 4-aminobutyrate aminotransferase antibody
      • ABAT antibody
      • FLJ17813 antibody
      • FLJ30272 antibody
      • GABA aminotransferase antibody
      • GABA AT antibody
      • GABA T antibody
      • GABA transaminase antibody
      • GABA transferase antibody
      • GABA-AT antibody
      • GABA-T antibody
      • GABAT antibody
      • GABT_HUMAN antibody
      • Gamma amino N butyrate transaminase antibody
      • Gamma-amino-N-butyrate transaminase antibody
      • hCG1984265 antibody
      • L AIBAT antibody
      • L-AIBAT antibody
      • LAIBAT antibody
      • mitochondrial antibody
      • NPD009 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat liver tissue sections labeling ABAT with Purified ab108249 at 1:1000 dilution (0.9 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

    • All lanes : Anti-ABAT antibody [EPR4433] (ab108249) at 1/10000 dilution (Purified)

      Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates with 5% NFDM /TBST
      Lane 2 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates with 5% NFDM /TBST
      Lane 3 : Mouse brain lysates with 5% NFDM /TBST
      Lane 4 : Rat brain lysates with 5% NFDM /TBST

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 56 kDa
      Observed band size: 50 kDa (why is the actual band size different from the predicted?)

    • ab108249 (purified) at 1:40 dilution (2µg) immunoprecipitating ABAT in HepG2 whole cell lysate.
      Lane 1 (input): HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate 10µg
      Lane 2 (+): ab108249 & HepG2 whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab108249 in HepG2 whole cell lysate
      For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used as the secondary antibody at 1:1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebrum tissue sections labeling ABAT with Purified ab108249 at 1:1000 dilution (0.9 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling ABAT with Purified ab108249 at 1:1000 dilution (0.9 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

    • Unpurified ab108249, at 1/250 dilution, staining ABAT in Human liver tissue by immunohistochemistry.

    • All lanes : Anti-ABAT antibody [EPR4433] (ab108249) (unpurified)

      Lane 1 : HepG2 cell lysate
      Lane 2 : SH-SY5Y cell lysate
      Lane 3 : Human fetal liver tissue lysate
      Lane 4 : rat brain tissue lysate
      Lane 5 : rat kidney tissue lysate
      Lane 6 : mouse brain tissue lysate

      Predicted band size: 56 kDa

    参考文献

    This product has been referenced in:
    • Chen L  et al. Involvement of upregulation of miR-210 in a rat epilepsy model. Neuropsychiatr Dis Treat 12:1731-7 (2016). WB . Read more (PubMed: 27471387) »
    • De Ita-Pérez D  et al. Synchronization by food access modifies the daily variations in expression and activity of liver GABA transaminase. Biomed Res Int 2014:590581 (2014). WB ; Rat . Read more (PubMed: 24809054) »

    See all 3 Publications for this product

    レビューと Q&A

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Sheep Tissue lysate - whole (Liver tissue)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    Liver tissue
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Feb 14 2018

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (breast cancer)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    breast cancer
    Blocking step
    Milk as blocking agent for 1 hour(s) and 23 minute(s) · Concentration: 0.5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Dec 05 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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