Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (HRP) (ab99697)
Key features and details
- Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (HRP)
- Conjugation: HRP
- Host species: Mouse
- Isotype: IgG2b
- Suitable for: IHC-P, ELISA, WB
Related conjugates and formulations
製品の概要
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製品名
Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (HRP)
IgG Light chain 二次抗体 製品一覧 -
由来種
Mouse -
ターゲット生物種
Rabbit -
特異性
ab99697 reacts with the light chain of native Rabbit IgG primary antibodies and does not bind to the reduced and denatured Rabbit IgG heavy chain band (50kD). -
アプリケーション
適用あり: IHC-P, ELISA, WBmore details -
免疫原
Full length protein corresponding to Rabbit IgG Light chain. Rabbit Light Chain
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標識
HRP
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.4
Constituents: 50% Glycerol (glycerin, glycerine), 50% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
SB62a -
アイソタイプ
IgG2b -
軽鎖の種類
lambda -
研究分野
関連製品
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Alternative Versions
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Related Products
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab99697の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/8000.
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ELISA |
1/4000 - 1/8000.
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|
WB |
Use at an assay dependent concentration.
Note: This antibody is not recommended for sensitive or quantitative detection of the reduced and denatured Rabbit Light Chains on Western Blots. |
特記事項 |
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IHC-P
1/8000. |
ELISA
1/4000 - 1/8000. |
WB
Use at an assay dependent concentration. Note: This antibody is not recommended for sensitive or quantitative detection of the reduced and denatured Rabbit Light Chains on Western Blots. |
画像
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IHC image of beta actin staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8227, 3µg/ml overnight at +4°C. An HRP-conjugated secondary (ab99697, 1/8000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.
The inset negative control image is secondary-only at 1/4000 dilution.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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Western blot showing that Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697) specifically binds to light-chains
Left blot: Coomassie stain showing the rabbit heavy (~50kDa) and light-chains (~25 kDa)
Lane 1: 10 µg rabbit monoclonal was loaded
Lane 2: 5 µg rabbit monoclonal was loaded
Lane 3: 2 µg rabbit monoclonal was loadedRight blot: WB using Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697) as secondary antibody at 1/10,000
Lane 1: 1 µg rabbit monoclonal was loaded
Lane 2: 0.5 µg rabbit monoclonal was loaded
Lane 3: 0.25 µg rabbit monoclonal was loaded -
All lanes : Anti-beta Actin antibody - Loading Control (ab8227) at 1 µg/ml
Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252)Lysates/proteins at 10 µg per lane.
Secondary
Lanes 1 - 2 : Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697) at 1/2000 dilution
Lanes 3 - 4 : Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697) at 1/10000 dilution
Lanes 5 - 6 : Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697) at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions. -
LAP2 alpha was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to LAP2 alpha (ab5162)and 50µl of protein G magnetic beads (lane 1). The antibody was incubated with the Protein G beads for 10min under agitation. No antibody was added to the control (lane 2). Hela whole cell extract diluted in RIPA buffer was added to each sample and incubated for 10min under agitation. Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab5162. Secodary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Bands: 83kDa: LAP2 alpha. -
Catalase was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Catalase (ab1877) and 50µl of protein G magnetic beads (lane 1). The antibody was incubated with the Protein G beads for 10min under agitation. No antibody was added to the control (lane 2). Hela whole cell extract diluted in RIPA buffer was added to each sample and incubated for 10min under agitation. Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab1877. Secodary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697). Bands: 60kDa: Catalase
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Rad21 was immunoprecipitated using 0.5mg Hela whole cell extract, 5ug of Rabbit polyclonal to Rad21and 50µl of protein G magnetic beads (lane 1). The antibody was incubated with the Protein G beads for 10min under agitation. No antibody was added to the control (lane 2). Hela whole cell extractdiluted in RIPA buffer was added to each sample and incubated for 10min under agitation. Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab42478. Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697) .. Band: 100kDa: Rad21; Non specific - 50kDa: We are unsure as to the identity of this extra band. Although the predicted band size is 75kDa based on Swiss-prot data, a band of 120kDa has been previously observed. Mol Cell Biol. 2002 Dec;22(23):8267-77 PMID: 12417729. Th
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Lane 1: Rabbit IgG loaded at 1ug/well
ab99697 diluted to 1:2000
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (78)
ab99697 は 78 報の論文で使用されています。
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