Goat Anti-Mouse IgG1 (HRP) (ab97240)
Key features and details
- Goat Anti-Mouse IgG1 (HRP)
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC, ELISA, IHC-P, WB
製品の概要
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製品名
Goat Anti-Mouse IgG1 (HRP)
IgG1 二次抗体 製品一覧 -
由来種
Goat -
ターゲット生物種
Mouse -
特異性
By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG1. Cross reactivity with other immunoglobulins is less than 2%. This antibody may cross react with IgG1 from other species. -
アプリケーション
適用あり: ICC, ELISA, IHC-P, WBmore details -
標識
HRP
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 6.8
Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Antiserum was solid phase adsorbed to ensure subclass specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP). -
特記事項(標識)
Molar enzyme/ antibody protein ratio is 4:1 -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97240の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC |
1/200 - 1/2000.
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ELISA |
1/10000 - 1/100000.
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|
IHC-P |
1/200 - 1/2000.
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|
WB | (1) |
1/2000 - 1/20000. Colorimetric method; 1/2000 - 1/20000 Chemiluminescence method.
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特記事項 |
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ICC
1/200 - 1/2000. |
ELISA
1/10000 - 1/100000. |
IHC-P
1/200 - 1/2000. |
WB
1/2000 - 1/20000. Colorimetric method; 1/2000 - 1/20000 Chemiluminescence method. |
画像
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IHC image of Histone H1.0 staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab11080, 7.5µg/ml overnight at +4°C. An HRP-conjugated secondary (ab97240, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.
The inset negative control image is secondary-only at 1/500 dilution.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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All lanes : Anti-Topoisomerase I antibody (ab58313)
Lane 1 : Products generated by incubation of hTop1 with [? -32P] end-labelled duplex DNA (CL25/CP25) incubated in the absence of CPT for 1 minute.
Lane 2 : Products generated by incubation of hTop1 with [? -32P] end-labelled duplex DNA (CL25/CP25) incubated in the absence of CPT for 2 minutes.
Lane 3 : Products generated by incubation of hTop1 with [? -32P] end-labelled duplex DNA (CL25/CP25) incubated in the absence of CPT for 4 minutes.
Lane 4 : Products generated by incubation of hTop1 with [? -32P] end-labelled duplex DNA (CL25/CP25) incubated in the absence of CPT for 8 minutes.
Lane 5 : Products generated by incubation of hTop1 with [? -32P] end-labelled duplex DNA (CL25/CP25) incubated in the presence of CPT for 1 minute.
Lane 6 : Products generated by incubation of hTop1 with [? -32P] end-labelled duplex DNA (CL25/CP25) incubated in the presence of CPT for 2 minutes.
Lane 7 : Products generated by incubation of hTop1 with [? -32P] end-labelled duplex DNA (CL25/CP25) incubated in the presence of CPT for 4 minutes.
Lane 8 : Products generated by incubation of hTop1 with [? -32P] end-labelled duplex DNA (CL25/CP25) incubated in the presence of CPT for 8 minutes.
Secondary
All lanes : Goat Anti-Mouse IgG1 (HRP) (ab97240)The band corresponding to the enzyme–substrate cleaved complex, has been indicated by an asterisk.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (126)
ab97240 は 126 報の論文で使用されています。
- Maniar R et al. Self-Renewing CD8+ T-cell Abundance in Blood Associates with Response to Immunotherapy. Cancer Immunol Res 11:164-170 (2023). PubMed: 36512052
- Chen S et al. Assembly of Immunogenic Protein Particles toward Advanced Synthetic Vaccines. Small 19:e2205819 (2023). PubMed: 36564365
- Weyant KB et al. A modular vaccine platform enabled by decoration of bacterial outer membrane vesicles with biotinylated antigens. Nat Commun 14:464 (2023). PubMed: 36709333
- Stoler-Barak L et al. B cell class switch recombination is regulated by DYRK1A through MSH6 phosphorylation. Nat Commun 14:1462 (2023). PubMed: 36927854
- Walker MWG et al. Novel molecular requirements for CRISPR RNA-guided transposition. Nucleic Acids Res 51:4519-4535 (2023). PubMed: 37078593