Anti-PUMA 抗体 (ab9643)
Key features and details
- Rabbit polyclonal to PUMA
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-PUMA antibody
PUMA 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PUMA -
由来種
Rabbit -
特異性
At least 2 isoforms are known to exist; this antibody will detect both isoforms. -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human PUMA aa 150-250 (C terminal).
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特記事項
Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
一次抗体 備考
Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab9643の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (9) |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 23 kDa. Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 23 kDa. Can be blocked with PUMA peptide (180/193) (ab9644). A lower band at approximately 16 kDa was detected in MOLT4 and U937 cells, which may represent the PUMA-beta form.
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ICC/IF |
Use a concentration of 1 µg/ml.
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特記事項 |
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 23 kDa. Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 23 kDa. Can be blocked with PUMA peptide (180/193) (ab9644). A lower band at approximately 16 kDa was detected in MOLT4 and U937 cells, which may represent the PUMA-beta form. |
ICC/IF
Use a concentration of 1 µg/ml. |
ターゲット情報
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機能
Essential mediator of p53-dependent and p53-independent apoptosis. -
組織特異性
Ubiquitously expressed. -
配列類似性
Belongs to the Bcl-2 family. -
細胞内局在
Mitochondrion. Localized to the mitochondria in order to induce cytochrome c release. - Information by UniProt
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参照データベース
- Entrez Gene: 27113 Human
- Entrez Gene: 170770 Mouse
- Omim: 605854 Human
- SwissProt: Q9BXH1 Human
- SwissProt: Q99ML1 Mouse
- Unigene: 467020 Human
- Unigene: 7660 Mouse
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別名
- BBC 3 antibody
- Bbc3 antibody
- BBC3_HUMAN antibody
see all
画像
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All lanes : Anti-PUMA antibody (ab9643) at 2 µg/ml
Lane 1 : HEK293 cells were transfected with control siRNAs with control siRNAs
Lane 2 : HEK293 cells were transfected with PUMA siRNAs with PUMA siRNA
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilutionBeta-actin (1 μg/mL) and GAPDH (0.02 μg/mL). Incubation time: 1 hour at Room Temperature in 5% NFDM/TBST.
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Immunofluorescent analysis of 4% paraformaldehydefixed K562 cells labeling PUMA with ab9643 at 2 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). Image showing cytosol staining on K562 cells.
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ICC/IF image of ab9643 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9643, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunocytochemical analysis of K562 cells labeling PUMA with ab9643 at 1 μg/mL. Cells were fixed with formaldehyde and blocked with 10% serum for 1 hour at room temperature. Antigen retrieval was by heat mediation with a citrate buffer (pH 6.0). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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All lanes : Anti-PUMA antibody (ab9643) at 2 µg/ml
Lane 1 : K562 cell lysate
Lane 2 : NIH3T3 cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution -
Anti-PUMA antibody (ab9643) at 1/1000 dilution + HeLa whole cell lysate
Secondary
Alexa Fluor 680-conjugated goat anti-rabbit IgG polyclonal at 1/1 dilution
Observed band size: 20 kDa why is the actual band size different from the predicted?
Additional bands at: 26 kDa (possible non-specific binding), 42 kDa (possible non-specific binding), 50 kDa (possible non-specific binding)
Exposure time: 5 secondsBlocked with 5% milk for 1 hour.
Incubated with the primary antibody for 18 hours.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (121)
ab9643 は 121 報の論文で使用されています。
- Wei Z et al. Inhibition of secretory leukocyte protease inhibitor (SLPI) promotes the PUMA-mediated apoptosis and chemosensitivity to cisplatin in colorectal cancer cells. Discov Oncol 14:1 (2023). PubMed: 36595102
- Young A et al. Targeting the Pro-survival Protein BCL-2 to Prevent Breast Cancer. Cancer Prev Res (Phila) 15:3-10 (2022). PubMed: 34667127
- Quintero-Espinosa DA et al. Phenolic-rich extract of avocado Persea americana (var. Colinred) peel blunts paraquat/maneb-induced apoptosis through blocking phosphorylation of LRRK2 kinase in human nerve-like cells. Environ Toxicol 37:660-676 (2022). PubMed: 34897981
- Chen H et al. Lysophosphatidylcholine disrupts cell adhesion and induces anoikis in hepatocytes. FEBS Lett 596:510-525 (2022). PubMed: 35043979
- Lai TC et al. Combined exposure to fine particulate matter and high glucose aggravates endothelial damage by increasing inflammation and mitophagy: the involvement of vitamin D. Part Fibre Toxicol 19:25 (2022). PubMed: 35351169