Anti-Pan3 抗体 (ab88642)
Key features and details
- Rabbit polyclonal to Pan3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Pan3 antibody -
製品の詳細
Rabbit polyclonal to Pan3 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human Pan3 aa 400-500 conjugated to keyhole limpet haemocyanin.
(Peptide available asab102602) -
ポジティブ・コントロール
- This antibody gave a positive signal in the following lysates: HeLa Whole Cell; HepG2 Whole Cell; MOLT4 Whole Cell; Jurkat Whole Cell; Human Liver Tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab88642の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 82 kDa).
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 82 kDa). |
ターゲット情報
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機能
Functions in cytoplasmic mRNA decay. As part of the Pan nuclease complex, recruits polyadenylate-binding protein which in turn stimulates PAN2 nuclease activity. -
配列類似性
Belongs to the protein kinase superfamily.
Contains 1 protein kinase domain. -
ドメイン
The protein kinase domain is predicted to be catalytically inactive. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 255967 Human
- Entrez Gene: 72587 Mouse
- Entrez Gene: 360760 Rat
- SwissProt: Q58A45 Human
- SwissProt: Q640Q5 Mouse
- Unigene: 645015 Human
- Unigene: 320469 Mouse
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別名
- hPan3 antibody
- PAB dependent poly(A) specific ribonuclease subunit 3 antibody
- PAB-dependent poly(A)-specific ribonuclease subunit 3 antibody
see all
画像
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All lanes : Anti-Pan3 antibody (ab88642) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 5 : Human liver tissue lysate - total protein (ab29889)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 82 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Additional bands at: 160 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab88642 は 2 報の論文で使用されています。
- Liu J et al. YTHDF2/3 Are Required for Somatic Reprogramming through Different RNA Deadenylation Pathways. Cell Rep 32:108120 (2020). PubMed: 32905781
- Yang S et al. USP52 acts as a deubiquitinase and promotes histone chaperone ASF1A stabilization. Nat Commun 9:1285 (2018). PubMed: 29599486