Recombinant Human 14-3-3 zeta protein (ab85268)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Suitable for: SDS-PAGE, WB
製品の詳細
-
製品名
Recombinant Human 14-3-3 zeta protein
14-3-3 zeta タンパク質・ペプチド 製品一覧 -
精製度
> 95 % SDS-PAGE.
Purity was determined to be >95% by densitometry. -
発現系
Escherichia coli -
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
-
生物種
Human
-
関連製品
-
Related Products
特性
Our Abpromise guarantee covers the use of ab85268 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
アプリケーション
SDS-PAGE
Western blot
-
製品の状態
Liquid -
Concentration information loading...
前処理および保存
-
保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 25% Glycerol (glycerin, glycerine), 0.29% Sodium chloride
関連情報
-
別名
- 14 3 3 delta
- 14 3 3 protein zeta/delta
- 14 3 3 protein/cytosolic phospholipase A2
see all -
機能
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. -
配列類似性
Belongs to the 14-3-3 family. -
翻訳後修飾
The delta, brain-specific form differs from the zeta form in being phosphorylated (By similarity). Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53. This phosphorylation appears to be activated by sphingosine. Phosphorylation on Thr-232; inhibits binding of RAF1. -
細胞内局在
Cytoplasm. Melanosome. Located to stage I to stage IV melanosomes. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab85268 は 1 報の論文で使用されています。
- Liu QW et al. N-linked glycoproteomic profiling in esophageal squamous cell carcinoma. World J Gastroenterol 28:3869-3885 (2022). PubMed: 36157541