Recombinant human TGF beta 2 protein (Active) (ab84070)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 98% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: HPLC, Functional Studies, SDS-PAGE, WB
Achieve higher consistency and quality standards with a premium grade bioactive protein
- High batch-to-batch consistency
- Optimal bioactivity
- Guaranteed identical to human native proteins
- >95% purity
- Ultra-low endotoxin levels: <0.005 Eu/µg
- Carrier and tag free
製品の詳細
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製品名
Recombinant human TGF beta 2 protein (Active)
TGF beta 2 タンパク質・ペプチド 製品一覧 -
生理活性
Determined by its ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells.
The ED50 was found to be = 0.2 ng/ml, corresponding to a specific activity of = 5 x 106 units/mg.
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精製度
> 98 % SDS-PAGE.
> 98% HPLC analyses. -
エンドトキシン・レベル
< 1.000 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
ALDAAYCFRN VQDNCCLRPL YIDFKRDLGW KWIHEPKGYN ANFCAGACPY LWSSDTQHSR VLSLYNTINP EASASPCCVS QDLEPLTILY YIGKTPKIEQ LSNMIVKSCK CS -
予測される分子量
25 kDa -
領域
303 to 414
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特性
Our Abpromise guarantee covers the use of ab84070 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
HPLC
Functional Studies
SDS-PAGE
Western blot
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Stable for 12 months at -20°C.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Reconstitute in water to 0.1-1.0 mg/ml. Store at 2°C to 8°C for up to 1 week or prepare for extended storage. After initial reconstitution, further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA). Store working aliquots at -20°C to -80°C.
関連情報
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別名
- BSC-1 cell growth inhibitor
- BSC1 cell growth inhibitor
- Cetermin
see all -
機能
TGF-beta 2 has suppressive effects on interleukin-2 dependent T-cell growth. -
関連疾患
A chromosomal aberration involving TGFB2 is found in a family with Peters anomaly. Translocation t(1;7)(q41;p21) with HDAC9.
Loeys-Dietz syndrome 4
Defects in TGFB2 may be a cause of non-syndromic aortic disease (NSAD). NSAD is a frequently asymptomatic but potentially lethal disease characterized by thoracic aortic aneurysms and dissections without additional syndromic features. -
配列類似性
Belongs to the TGF-beta family. -
翻訳後修飾
The precursor is cleaved into mature TGF-beta-2 and LAP, which remains non-covalently linked to mature TGF-beta-2 rendering it inactive. -
細胞内局在
Secreted. - Information by UniProt
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プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (8)
ab84070 は 8 報の論文で使用されています。
- Li Q et al. Naringenin inhibits autophagy and epithelial-mesenchymal transition of human lens epithelial cells by regulating the Smad2/3 pathway. Drug Dev Res 83:389-396 (2022). PubMed: 34402084
- Spurlin JW et al. BMP3 inhibits TGFβ2-mediated myofibroblast differentiation during wound healing of the embryonic cornea. NPJ Regen Med 7:36 (2022). PubMed: 35879352
- Jie X et al. USP9X-mediated KDM4C deubiquitination promotes lung cancer radioresistance by epigenetically inducing TGF-ß2 transcription. Cell Death Differ 28:2095-2111 (2021). PubMed: 33558705
- Finger AM et al. Intercellular coupling between peripheral circadian oscillators by TGF-ß signaling. Sci Adv 7:N/A (2021). PubMed: 34301601
- Corbet C et al. TGFß2-induced formation of lipid droplets supports acidosis-driven EMT and the metastatic spreading of cancer cells. Nat Commun 11:454 (2020). PubMed: 31974393
- Babushkina A & Lwigale P Periocular neural crest cell differentiation into corneal endothelium is influenced by signals in the nascent corneal environment. Dev Biol 465:119-129 (2020). PubMed: 32697973
- Tran DQ et al. GARP (LRRC32) is essential for the surface expression of latent TGF-beta on platelets and activated FOXP3+ regulatory T cells. Proc Natl Acad Sci U S A 106:13445-50 (2009). PubMed: 19651619
- O'Riordan E et al. Chronic NOS inhibition actuates endothelial-mesenchymal transformation. Am J Physiol Heart Circ Physiol 292:H285-94 (2007). PubMed: 16963618