HEK-293 whole cell lysate (ab7902)
製品の概要
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製品名
HEK-293 whole cell lysate
HEK293 ライゼート 製品一覧 -
特記事項
Cell line: HEK293 (Human embryonic kidney cell line).
Growth media: RPMI and 10% FBS (Fetal bovine serum).293 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (0.045 M Tris-HCl pH 6.8, 10% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue), containing 0.05 M DTT.
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アプリケーション
適用あり: WBmore details
製品の特性
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Mycoplasma free
Yes -
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Constituent: 100% SDS Sample Buffer -
Concentration information loading...
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ライゼート 備考
293 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (0.045 M Tris-HCl pH 6.8, 10% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue), containing 0.05 M DTT.
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研究分野
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背景
Human Embryonic Kidney (HEK) 293 cells were originally derived from human embryonic kidney and subsequently demonstrated to be a useful cell type to produce adenovirus, other viral vectors, and effectively glycosylated human recombinant proteins. They are therefore used for virology studies and transfected to express recombinant proteins for studies in a number of research fields.
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab7902の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent dilution.
293 cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel. |
特記事項 |
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WB
Use at an assay dependent dilution. 293 cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel. |
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab7902 は 4 報の論文で使用されています。
- Sun T et al. Salvianolic acid B activates chondrocytes autophagy and reduces chondrocyte apoptosis in obese mice via the KCNQ1OT1/miR-128-3p/SIRT1 signaling pathways. Nutr Metab (Lond) 19:53 (2022). PubMed: 35922815
- Ferchaud-Roucher V et al. Maternal obesity results in decreased syncytiotrophoblast synthesis of palmitoleic acid, a fatty acid with anti-inflammatory and insulin-sensitizing properties. FASEB J 33:6643-6654 (2019). PubMed: 30811959
- Niittykoski M et al. Immunohistochemical Characterization and Sensitivity to Human Adenovirus Serotypes 3, 5, and 11p of New Cell Lines Derived from Human Diffuse Grade II to IV Gliomas. Transl Oncol 10:772-779 (2017). PubMed: 28797937
- Awais R et al. p63 is required beside p53 for PERP-mediated apoptosis in uveal melanoma. Br J Cancer 115:983-992 (2016). PubMed: 27584665