Anti-RKIP 抗体 [EPR2875Y] (ab76582)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2875Y] to RKIP
- Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-RKIP antibody [EPR2875Y]
RKIP 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR2875Y] to RKIP -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), IHC-P, WB, ICC/IFmore details
適用なし: IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human RKIP aa 50-150. The exact sequence is proprietary.
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ポジティブ・コントロール
- WB: HeLa, A549, HepG2, A431, mouse brain, mouse testis and rat brain cell lysates; IHC-P: human ovarian carcinoma and brain, mouse kidney, and rat testis tissues; ICC/IF: HepG2 cells; Flow Cyt (intra): HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR2875Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab76582の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
1/20.
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB | (3) |
1/1000 - 1/5000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
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ICC/IF | (2) |
1/50 - 1/100.
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特記事項 |
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Flow Cyt (Intra)
1/20. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
1/1000 - 1/5000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). |
ICC/IF
1/50 - 1/100. |
ターゲット情報
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機能
Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase.
HCNP may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. HCNP increases the production of choline acetyltransferase but not acetylcholinesterase. Seems to be mediated by a specific receptor. -
配列類似性
Belongs to the phosphatidylethanolamine-binding protein family. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 5037 Human
- Entrez Gene: 23980 Mouse
- Entrez Gene: 29542 Rat
- Omim: 604591 Human
- SwissProt: P30086 Human
- SwissProt: P70296 Mouse
- SwissProt: P31044 Rat
- Unigene: 433863 Human
see all -
別名
- Epididymis luminal protein 210 antibody
- Epididymis secretory protein Li 34 antibody
- Epididymis secretory protein Li 96 antibody
see all
画像
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All lanes : Anti-RKIP antibody [EPR2875Y] (ab76582) at 1/1000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : A549 (Human lung carcinoma epithelial cell) whole cell lysates
Lane 3 : Mouse brain lysates
Lane 4 : Mouse testis lysates
Lane 5 : Rat brain lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa -
Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling RKIP with purified ab76582 at 1:50 dilution (2.6 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat testis tissue sections labeling RKIP with purified ab76582 at 1/500 dilution (0.26 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling RKIP (red) with purified ab76582 at a 1/20 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluorr® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling RKIP with purified ab76582 at 1/500 dilution (0.26 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue sections labeling RKIP with purified ab76582 at 1/500 dilution (0.26 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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All lanes : Anti-RKIP antibody [EPR2875Y] (ab76582) at 1/5000 dilution (unpurified)
Lane 1 : HeLa cell lysate
Lane 2 : A549 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa -
Staining of formalin-fixed, paraffin-embedded human brain tissue using 1/250 ab76582 (unpurified).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Anti-RKIP antibody [EPR2875Y] (ab76582) at 1/5000 dilution (unpurified) +
Recombinant Human RKIP protein (ab87892) at 0.1 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 21 kDa
Exposure time: 30 seconds
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (18)
ab76582 は 18 報の論文で使用されています。
- Wang W et al. Dioscorea nipponica Makino Relieves Ovalbumin-Induced Asthma in Mice through Regulating RKIP-Mediated Raf-1/MEK/MAPK/ERK Signaling Pathway. Biomed Res Int 2022:8077058 (2022). PubMed: 35757465
- Ren J et al. Key ferroptosis-related genes in abdominal aortic aneurysm formation and rupture as determined by combining bioinformatics techniques. Front Cardiovasc Med 9:875434 (2022). PubMed: 36017103
- Jiao H et al. Traditional Chinese Formula Xiaoyaosan Alleviates Depressive-Like Behavior in CUMS Mice by Regulating PEBP1-GPX4-Mediated Ferroptosis in the Hippocampus. Neuropsychiatr Dis Treat 17:1001-1019 (2021). PubMed: 33854318
- You H et al. Sight and switch off: Nerve density visualization for interventions targeting nerves in prostate cancer. Sci Adv 6:eaax6040 (2020). PubMed: 32076639
- Penas C et al. RKIP Regulates Differentiation-Related Features in Melanocytic Cells. Cancers (Basel) 12:N/A (2020). PubMed: 32503139