Anti-PPP2R3B 抗体 (ab72027)
Key features and details
- Rabbit polyclonal to PPP2R3B
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PPP2R3B antibody
PPP2R3B 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PPP2R3B -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Rhesus monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide from a region between residue 350 and 400 of human PPP2R3B, NP_037371.2.
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ポジティブ・コントロール
- Whole cell lysate from Hela and 293T cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab72027の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | ||
IP |
特記事項 |
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WB: 1/2000 - 1/10000. Detects bands of approximately 65 and 200 kDa (predicted molecular weight: 65 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能
The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. -
配列類似性
Contains 1 EF-hand domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 28227 Human
- Omim: 300339 Human
- SwissProt: Q9Y5P8 Human
- Unigene: 124942 Human
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別名
- NY REN 8 antibody
- NY REN 8 antigen antibody
- P2R3B_HUMAN antibody
see all
画像
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All lanes : Anti-PPP2R3B antibody (ab72027) at 0.1 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Predicted band size: 65 kDa
Observed band size: 65 kDa
Additional bands at: 200 kDa. We are unsure as to the identity of these extra bands. -
Immunoprecipitation/ Western Blot of PPP2R3B.
Lane 1: ab72027 at 3µg/mg whole cell lysate. Lane 2: Control IgG.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded. For western blot detection step ab72027 was used at 1 µg/ml.
Chemiluminescence with an exposure time of 30 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab72027 は論文での使用が確認できていません。