Anti-PKC zeta (phospho T560) 抗体 [EP2037AY] (ab62372)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2037AY] to PKC zeta (phospho T560)
- Suitable for: WB, IHC-P, Dot blot
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-PKC zeta (phospho T560) antibody [EP2037AY]
PKC zeta 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP2037AY] to PKC zeta (phospho T560) -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, Dot blotmore details
適用なし: Flow Cyt or ICC/IF -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human PKC zeta aa 550-650 (phospho T560). The exact sequence is proprietary.
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ポジティブ・コントロール
- WB: HeLa cell lysates. IHC-P: Human brain tissue and human breast ductal carcinoma tissue.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP2037AY -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab62372の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa).
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IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Dot blot |
1/1000.
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特記事項 |
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WB
1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa). |
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Dot blot
1/1000. |
ターゲット情報
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機能
This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Phosphorylates 'Ser-311' of RELA subunit of NF-kappa-B.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Subunit of a quaternary complex that plays a central role in epithelial cell polarization. -
組織特異性
Expressed in brain, and to a lesser extent in lung, kidney and testis. -
配列類似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 OPR domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain. -
ドメイン
The OPR domain mediates mutually exclusive interactions with SQSTM1 and PARD6B.
The C1 domain does not bind the diacylglycerol (DAG). -
翻訳後修飾
CDH5 is required for its phosphorylation at Thr-410. -
細胞内局在
Cytoplasm. Endosome. Cell junction. In the retina, localizes in the terminals of the rod bipolar cells (By similarity). Associates with endosomes. Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction. - Information by UniProt
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参照データベース
- Entrez Gene: 5590 Human
- Entrez Gene: 18762 Mouse
- Entrez Gene: 25522 Rat
- Omim: 176982 Human
- SwissProt: Q05513 Human
- SwissProt: Q02956 Mouse
- SwissProt: P09217 Rat
- Unigene: 496255 Human
see all -
別名
- 14-3-3-zetaisoform antibody
- AI098070 antibody
- aPKCzeta antibody
see all
画像
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All lanes : Anti-PKC zeta (phospho T560) antibody [EP2037AY] (ab62372) at 1/1000 dilution
Lane 1 :Recombinant human PKC alpha protein (Active) (ab55672)
Lane 2 :Recombinant human PKC beta 1 protein (ab60840)
Lane 3 :Recombinant human PKC beta 2 protein (ab60841)
Lane 4 :Recombinant human PKC gamma protein (ab60842)
Lane 5 :Recombinant human PKC delta protein (ab60844)
Lane 6 :Recombinant human PKC epsilon protein (ab60847)
Lane 7 :Recombinant human PKC zeta protein (ab60848)
Lane 8 :Recombinant human PKC eta protein (ab60849)
Lane 9 :Recombinant human PKC theta/PRKCQ protein (ab56641)
Lane 10 :Recombinant human PKC iota protein (ab60850)
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 68 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
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All lanes : Anti-PKC zeta (phospho T560) antibody [EP2037AY] (ab62372) at 1/20000 dilution
Lane 1 : HeLa cell lysate - treated with Calyculin A
Lane 2 : HeLa cell lysate - treated with Calyculin A and lambda phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 68 kDa
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM/TBST.
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ab62372 at 1/100 dilution staining PKC zeta in human brain by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Dot blot analysis of PKC zeta (pT560) peptide (Lane 1) and PKC zeta non-phospho peptide (Lane 2) labelling PKC zeta (pT560) with ab62372 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
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All lanes : Anti-PKC zeta (phospho T560) antibody [EP2037AY] (ab62372)
Lane 1 : Hela Cell Lysate (untreated with Calyculin A)
Lane 2 : Hela Cell Lysate (Treated with Calyculin A)
Predicted band size: 68 kDa -
ab62372 at 1/100 dilution staining PKC zeta in human breast ductal carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (22)
ab62372 は 22 報の論文で使用されています。
- Burr SD et al. Rap1a Activity Elevated the Impact of Endogenous AGEs in Diabetic Collagen to Stimulate Increased Myofibroblast Transition and Oxidative Stress. Int J Mol Sci 23:N/A (2022). PubMed: 35562872
- Liu S et al. G3BP1 regulates breast cancer cell proliferation and metastasis by modulating PKCζ. Front Genet 13:1034889 (2022). PubMed: 36330442
- Burr SD & Stewart JA Rap1a Overlaps the AGE/RAGE Signaling Cascade to Alter Expression of a-SMA, p-NF-?B, and p-PKC-? in Cardiac Fibroblasts Isolated from Type 2 Diabetic Mice. Cells 10:N/A (2021). PubMed: 33806572
- Chow RY et al. MTOR promotes basal cell carcinoma growth through atypical PKC. Exp Dermatol 30:358-366 (2021). PubMed: 33617094
- Tapia R et al. Enteropathogenic Escherichia coli (EPEC) Recruitment of PAR Polarity Protein Atypical PKC? to Pedestals and Cell-Cell Contacts Precedes Disruption of Tight Junctions in Intestinal Epithelial Cells. Int J Mol Sci 21:N/A (2020). PubMed: 31947656