Anti-PPP1A/PPP1CA (phospho T320) 抗体 [EP1512Y] (ab62334)
Key features and details
- Rabbit monoclonal [EP1512Y] to PPP1A/PPP1CA (phospho T320)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
製品の概要
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製品名
Anti-PPP1A/PPP1CA (phospho T320) antibody [EP1512Y]
PPP1A/PPP1CA 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP1512Y] to PPP1A/PPP1CA (phospho T320) -
由来種
Rabbit -
特異性
This antibody detects PP1 alpha phosphorylated at Threonine 320.
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アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt or ICC/IF -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Xenopus laevis -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: HeLa cell lysate. IHC-P: Human muscle tissue.
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特記事項
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP1512Y -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab62334の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/1000 - 1/10000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. -
配列類似性
Belongs to the PPP phosphatase family. PP-1 subfamily. -
細胞内局在
Cytoplasm. Nucleus. Nucleus > nucleoplasm. Nucleus > nucleolus. Primarily nuclear and largely excluded from the nucleolus. Highly mobile in cells and can be relocalized through interaction with targeting subunits. NOM1 plays a role in targeting this protein to the nucleolus. In the presence of PPP1R8 relocalizes from the nucleus to nuclear speckles. - Information by UniProt
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参照データベース
- Entrez Gene: 5499 Human
- Entrez Gene: 19045 Mouse
- Entrez Gene: 24668 Rat
- Entrez Gene: 379914 Xenopus laevis
- Omim: 176875 Human
- SwissProt: P62136 Human
- SwissProt: P62137 Mouse
- SwissProt: P62138 Rat
see all -
別名
- Alpha isoform serine threonine protein phosphatase PP1alpha 1 catalytic subunit antibody
- Catalytic subunit antibody
- EC 3.1.3.16 antibody
see all
画像
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All lanes : Anti-PPP1A/PPP1CA (phospho T320) antibody [EP1512Y] (ab62334)
Lane 1 : HeLa cell lysate (untreated)
Lane 2 : HeLa cell lysate (treated with Calyculin A)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugate at 1/2000 dilution
Predicted band size: 38 kDa
Observed band size: 38 kDa
The bottom image shows beta Tubulin as a positive control. -
ab62334 (1/100 - 1/250 dilution) staining human PPP1A/PPP1CA in human muscle tissue by immunohistochemistry using paraffin embedded tissue.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (20)
ab62334 は 20 報の論文で使用されています。
- Li J et al. ODF2L acts as a synthetic lethal partner with WEE1 inhibition in epithelial ovarian cancer models. J Clin Invest 133:N/A (2023). PubMed: 36378528
- Tavernier N et al. Bora phosphorylation substitutes in trans for T-loop phosphorylation in Aurora A to promote mitotic entry. Nat Commun 12:1899 (2021). PubMed: 33771996
- Kamenz J et al. Bistable, Biphasic Regulation of PP2A-B55 Accounts for the Dynamics of Mitotic Substrate Phosphorylation. Curr Biol 31:794-808.e6 (2021). PubMed: 33357450
- Lin R et al. Persistently Elevated mTOR Complex 1-S6 Kinase 1 Disrupts DARPP-32-Dependent D1 Dopamine Receptor Signaling and Behaviors. Biol Psychiatry 89:1058-1072 (2021). PubMed: 33353667
- Feizbakhsh O et al. A Peak of H3T3 Phosphorylation Occurs in Synchrony with Mitosis in Sea Urchin Early Embryos. Cells 9:N/A (2020). PubMed: 32272587