Anti-CD68 抗体 [FA-11] (ab53444)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [FA-11] to CD68
- Suitable for: ICC/IF, Flow Cyt (Intra)
- Reacts with: Mouse
Related conjugates and formulations
製品の概要
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製品名
Anti-CD68 antibody [FA-11]
CD68 一次抗体 製品一覧 -
製品の詳細
Rat monoclonal [FA-11] to CD68 -
由来種
Rat -
アプリケーション
適用あり: ICC/IF, Flow Cyt (Intra)more details
適用なし: IHC-Fr -
種交差性
交差種: Mouse -
免疫原
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Flow Cyt: RAW 264.7 cells ICC/IF: RAW 264.7
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
FA-11 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Alternative Versions
- Alexa Fluor® 405 Anti-CD68 antibody [FA-11] (ab199571)
- Alexa Fluor® 488 Anti-CD68 antibody [FA-11] (ab201844)
- Alexa Fluor® 647 Anti-CD68 antibody [FA-11] (ab201845)
- HRP Anti-CD68 antibody [FA-11] (ab216526)
- PE Anti-CD68 antibody [FA-11] (ab216701)
- PerCP Anti-CD68 antibody [FA-11] (ab220509)
- APC Anti-CD68 antibody [FA-11] (ab221251)
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab53444の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | (1) |
1/50.
|
Flow Cyt (Intra) |
1/100.
Membrane permeabilisation is required for this application. ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
特記事項 |
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ICC/IF
1/50. |
Flow Cyt (Intra)
1/100. Membrane permeabilisation is required for this application. ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. -
組織特異性
Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites. -
配列類似性
Belongs to the LAMP family. -
翻訳後修飾
N- and O-glycosylated. -
細胞内局在
Cell membrane and Endosome membrane. Lysosome membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 12514 Mouse
- SwissProt: P31996 Mouse
- Unigene: 15819 Mouse
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別名
- CD 68 antibody
- CD68 antibody
- CD68 antigen antibody
see all
画像
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RAW 264.7 cells were fixed in 4% PFA and permeabilized with 0.1% Triton X-100. Primary antibody, ab53444 at 1:50 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti-Rat secondary antibody (ab150157) at 1/1000 dilution at RT for 45 min. ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab53444 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI. Confocal image showing cytoplasmic staining in RAW 264.7 cell line.
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Flow Cytometry analysis of 4% paraformaldehyde-fixed RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling CD68 with AB53444 at a 1/100 dilution (1 μg) (Red) compared to Rat monoclonal IgG - Isotype Control (Black) and cells without incubation with primary antibody and secondary antibody (Blue). Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (256)
ab53444 は 256 報の論文で使用されています。
- Choi C et al. DSCR1 deficiency ameliorates the Aβ pathology of Alzheimer's disease by enhancing microglial activity. Life Sci Alliance 6:N/A (2023). PubMed: 36450444
- Paunel-Görgülü A et al. Peptidylarginine deiminase 4 deficiency in bone marrow cells prevents plaque progression without decreasing atherogenic inflammation in apolipoprotein E-knockout mice. Front Cardiovasc Med 9:1046273 (2022). PubMed: 36465436
- Cousin N et al. Antibody-Mediated Delivery of VEGF-C Promotes Long-Lasting Lymphatic Expansion That Reduces Recurrent Inflammation. Cells 12:N/A (2022). PubMed: 36611965
- Zhou T et al. miR-204-containing exosomes ameliorate GVHD-associated dry eye disease. Sci Adv 8:eabj9617 (2022). PubMed: 35020440
- Ozaki E et al. Minocycline suppresses disease-associated microglia (DAM) in a model of photoreceptor cell degeneration. Exp Eye Res 217:108953 (2022). PubMed: 35090890