Anti-Claudin 2 抗体 (ab53032)
Key features and details
- Rabbit polyclonal to Claudin 2
- Suitable for: ICC/IF, ELISA, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Claudin 2 antibody
Claudin 2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Claudin 2 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, ELISA, IHC-P, WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat -
免疫原
A synthetic peptide derived from human Claudin 2.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab53032 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab53032の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | (1) |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent concentration.
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IHC-P | (1) |
Use at an assay dependent concentration.
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WB |
1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
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特記事項 |
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ICC/IF
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
WB
1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa). |
ターゲット情報
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機能
Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity. -
配列類似性
Belongs to the claudin family. -
細胞内局在
Cell junction > tight junction. Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 9075 Human
- Entrez Gene: 12738 Mouse
- Entrez Gene: 300920 Rat
- Omim: 300520 Human
- SwissProt: P57739 Human
- SwissProt: O88552 Mouse
- Unigene: 522746 Human
- Unigene: 117068 Mouse
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別名
- Claudin-2 antibody
- Claudin2 antibody
- CLD2_HUMAN antibody
see all
画像
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using ab53032. The picture on the right is blocked with the synthesized peptide.
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All lanes : Anti-Claudin 2 antibody (ab53032)
Lane 1 : NIH/3T3 cells
Lane 2 : NIH/3T3 cells with blocking peptide
Predicted band size: 25 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (54)
ab53032 は 54 報の論文で使用されています。
- Xiang S & Xiao J Protective effects of syringic acid on inflammation, apoptosis and intestinal barrier function in Caco-2 cells following oxygen-glucose deprivation/reoxygenation-induced injury. Exp Ther Med 23:66 (2022). PubMed: 34934437
- Brice DP et al. Interleukin-27 Regulates the Function of the Gastrointestinal Epithelial Barrier in a Human Tissue-Derived Organoid Model. Biology (Basel) 11:N/A (2022). PubMed: 35336801
- Wang R et al. Gut microbiota regulates acute myeloid leukaemia via alteration of intestinal barrier function mediated by butyrate. Nat Commun 13:2522 (2022). PubMed: 35534496
- Reiner J et al. The dual GLP-1 and GLP-2 receptor agonist dapiglutide promotes barrier function in murine short bowel. Ann N Y Acad Sci 1514:132-141 (2022). PubMed: 35580981
- He L et al. Glutamine in suppression of lipopolysaccharide-induced piglet intestinal inflammation: The crosstalk between AMPK activation and mitochondrial function. Anim Nutr 10:137-147 (2022). PubMed: 35663373