Anti-MMP14 抗体 - Hinge region (ab38971)
Key features and details
- Rabbit polyclonal to MMP14 - Hinge region
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-MMP14 antibody - Hinge region
MMP14 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MMP14 - Hinge region -
由来種
Rabbit -
特異性
This antibody binds to MMP14, but does not cross react with the other MMP family members (MMP1, MMP2, MMP3, MMP9). -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human MMP14.
(Peptide available asab41091) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: 50% Glycerol -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab38971の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (3) |
1/2000 - 1/5000. Predicted molecular weight: 66 kDa. When used against the reduced protein this antibody identifies bands at 65 Kd and 63 Kd (the pro-form and active form) as well as activation/breakdown products.
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特記事項 |
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WB
1/2000 - 1/5000. Predicted molecular weight: 66 kDa. When used against the reduced protein this antibody identifies bands at 65 Kd and 63 Kd (the pro-form and active form) as well as activation/breakdown products. |
ターゲット情報
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機能
Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7. -
組織特異性
Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors. -
配列類似性
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
ドメイン
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
翻訳後修飾
The precursor is cleaved by a furin endopeptidase. -
細胞内局在
Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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参照データベース
- Entrez Gene: 4323 Human
- Omim: 600754 Human
- SwissProt: P50281 Human
- Unigene: 2399 Human
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別名
- Matrix metallopeptidase 14 (membrane inserted) antibody
- Matrix metalloproteinase 14 antibody
- Matrix metalloproteinase-14 antibody
see all
画像
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All lanes : Anti-MMP14 antibody - Hinge region (ab38971)
Lane 1 : Cell lysates from human dermal fibroblasts neonatal (no treatment)
Lane 2 : Cell lysates from human dermal fibroblasts neonatal (treated with II1beta)
Predicted band size: 66 kDa
Observed band size: 66 kDa -
Anti-MMP14 antibody - Hinge region (ab38971) at 1/1000 dilution + HT1080 whole cell lysate at 30 µg
Secondary
HRP-conjugated donkey anti-rabbit
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 66 kDa
Observed band size: 64 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
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All lanes : Anti-MMP14 antibody - Hinge region (ab38971) at 1/5000 dilution
Lane 1 : Whole cell lysate prepared from HT1080 cells
Lane 2 : Whole cell lysate prepared from human vascular smooth muscle cells
Lysates/proteins at 5 µg per lane.
Secondary
All lanes : HRP conjugated donkey anti-rabbit IgG at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 66 kDa
Observed band size: 63,65 kDa why is the actual band size different from the predicted?
Additional bands at: 20 kDa (possible non-specific binding)
Exposure time: 30 seconds
Blocked using 5% milk for 1 hour at 20°C.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (39)
ab38971 は 39 報の論文で使用されています。
- Zhang Y et al. Phenotypic characterization of primary cardiac fibroblasts from patients with HFpEF. PLoS One 17:e0262479 (2022). PubMed: 35015787
- Dole NS et al. Assessment of Osteocytes: Techniques for Studying Morphological and Molecular Changes Associated with Perilacunar/Canalicular Remodeling of the Bone Matrix. Methods Mol Biol 2230:303-323 (2021). PubMed: 33197021
- Hülsemann M et al. TC10 regulates breast cancer invasion and metastasis by controlling membrane type-1 matrix metalloproteinase at invadopodia. Commun Biol 4:1091 (2021). PubMed: 34531530
- Kegelman CD et al. YAP and TAZ Mediate Osteocyte Perilacunar/Canalicular Remodeling. J Bone Miner Res 35:196-210 (2020). PubMed: 31610061
- Bretaudeau C et al. AG-9, an Elastin-Derived Peptide, Increases In Vitro Oral Tongue Carcinoma Cell Invasion, through an Increase in MMP-2 Secretion and MT1-MMP Expression, in a RPSA-Dependent Manner. Biomolecules 11:N/A (2020). PubMed: 33396696