Anti-Bcl6 抗体 [EP529Y] (ab33901)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP529Y] to Bcl6
- Suitable for: IP, WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Bcl6 antibody [EP529Y]
Bcl6 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP529Y] to Bcl6 -
由来種
Rabbit -
アプリケーション
適用あり: IP, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human Bcl6 aa 650-750 (C terminal). The exact sequence is proprietary.
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ポジティブ・コントロール
- WB: Daudi and Ramos, HeLa and SH-SY5Y cell lysates. IP: Ramos cell lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP529Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab33901の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IP |
Use at an assay dependent concentration.
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WB |
1/500 - 1/2000. Detects a band of approximately 85-90 kDa (predicted molecular weight: 78 kDa).
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特記事項 |
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IP
Use at an assay dependent concentration. |
WB
1/500 - 1/2000. Detects a band of approximately 85-90 kDa (predicted molecular weight: 78 kDa). |
ターゲット情報
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機能
Transcriptional repressor which is required for germinal center formation and antibody affinity maturation. Probably plays an important role in lymphomagenesis. -
組織特異性
Expressed in germinal center T and B cells and in primary immature dendritic cells. -
関連疾患
Note=Chromosomal aberrations involving BCL6 may be a cause of B-cell non-Hodgkin lymphoma. Translocation t(3;14)(q27;q32); translocation t(3;22)(q27;q11) with immunoglobulin gene regions.
Note=A chromosomal aberration involving BCL6 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with POU2AF1/OBF1.
Note=A chromosomal aberration involving BCL6 may be a cause of lymphoma. Translocation t(3;4)(q27;p11) with ARHH/TTF. -
配列類似性
Contains 1 BTB (POZ) domain.
Contains 6 C2H2-type zinc fingers. -
ドメイン
The BTB domain mediates homodimerization. Its dimer interface mediates peptide binding such as to corepressors BCOR and NCOR2. -
翻訳後修飾
Phosphorylated by MAPK1 in response to antigen receptor activation. Phosphorylation induces its degradation by ubiquitin/proteasome pathway. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 604 Human
- Omim: 109565 Human
- SwissProt: P41182 Human
- Unigene: 478588 Human
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別名
- B cell CLL/lymphoma 6 antibody
- B cell lymphoma 6 protein antibody
- B-cell lymphoma 5 protein antibody
see all
画像
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Bcl6 was immunoprecipitated from Ramos (human Burkitt's lymphoma B lymphocyte), whole cell lysate with ab33901 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab33901 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1 (Input): Ramos (human Burkitt's lymphoma B lymphocyte), whole cell lysate, 10 μg
Lane 2 (+): Ramos whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab33901 in Ramos whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
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All lanes : Anti-Bcl6 antibody [EP529Y] (ab33901) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : BCL6 knockout HeLa cell lysate
Lane 3 : Ramos cell lysate
Lane 4 : SH-SY5Y cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 78 kDa
Observed band size: 78 kDaLanes 1-4: Merged signal (red and green). Green ab33901 observed at 78 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab33901 Anti-Bcl6 antibody [EP529Y] was shown to react with BCL6 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265410 (knockout cell lysate ab257178) was used. Wild-type and Bcl6 knockout samples were subjected to SDS-PAGE. ab33901 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-Bcl6 antibody [EP529Y] (ab33901) at 1/1000 dilution (Purified)
Lane 1 : Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysates prepared in 1%SDS Hot lysis method
Lane 2 : Ramos (Human Burkitt's lymphoma lymphoblast) whole cell lysates prepared in 1%SDS Hot lysis method
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 78 kDa
Exposure time: 10 secondsBlocking and diluting buffer: 5% NFDM/TBST.
This antibody works better in 1%SDS Hot Lysates in WB. For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).
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All lanes : Anti-Bcl6 antibody [EP529Y] (ab33901) at 1/1000 dilution (Purified)
Lane 1 : Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysates prepared in RIPA lysis method
Lane 2 : Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysates prepared in 1%SDS Hot lysis method
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 78 kDa
Exposure time: 3 minutesBlocking and diluting buffer: 5% NFDM/TBST.
This antibody works better in 1%SDS Hot Lysates in WB. For Lysate preparation protocol, please refer to the protocol here (downloadable copy).
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Anti-Bcl6 antibody [EP529Y] (ab33901) at 1/2000 dilution (Unpurified) + Daudi cell lysate
Predicted band size: 78 kDa
Observed band size: 85-90 kDa why is the actual band size different from the predicted?
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab33901 は 8 報の論文で使用されています。
- Zhou J & Liu R Upregulation of miR-144-3p expression attenuates glioma cell viability and invasion by targeting BCL6. Exp Ther Med 22:1157 (2021). PubMed: 34504602
- Jiao J et al. AID assists DNMT1 to attenuate BCL6 expression through DNA methylation in diffuse large B-cell lymphoma cell lines. Neoplasia 22:142-153 (2020). PubMed: 32062068
- Stein L et al. Immunophenotypic Characterization of Canine Splenic Follicular-Derived B-Cell Lymphoma. Vet Pathol 56:350-357 (2019). PubMed: 30636524
- Watatani Y et al. Molecular heterogeneity in peripheral T-cell lymphoma, not otherwise specified revealed by comprehensive genetic profiling. Leukemia 33:2867-2883 (2019). PubMed: 31092896
- Kuai Y et al. Wilms' tumor 1-associating protein plays an aggressive role in diffuse large B-cell lymphoma and forms a complex with BCL6 via Hsp90. Cell Commun Signal 16:50 (2018). PubMed: 30143009
- Zhu L et al. High expressions of BCL6 and Lewis y antigen are correlated with high tumor burden and poor prognosis in epithelial ovarian cancer. Tumour Biol 39:1010428317711655 (2017). PubMed: 28671040
- Sato F et al. Angioimmunoblastic T-cell lymphoma mice model. Leuk Res 37:21-7 (2013). PubMed: 23026775
- Hirata Y et al. BCL6 degradation caused by the interaction with the C-terminus of pro-HB-EGF induces cyclin D2 expression in gastric cancers. Br J Cancer 100:1320-9 (2009). WB, ICC/IF, IHC-P ; Human . PubMed: 19337254