Anti-STAT6 (phospho Y641) 抗体 (ab28829)
Key features and details
- Rabbit polyclonal to STAT6 (phospho Y641)
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-STAT6 (phospho Y641) antibody
STAT6 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to STAT6 (phospho Y641) -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human STAT6 (phospho Y641). Synthesized phosphopeptide derived from human STAT6 around the phosphorylation site of Tyrosine 641.
Database link: P42226 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab28829の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P | (6) |
1/50 - 1/100.
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WB | (1) |
1/500 - 1/1000. Predicted molecular weight: 94 kDa.
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特記事項 |
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IHC-P
1/50 - 1/100. |
WB
1/500 - 1/1000. Predicted molecular weight: 94 kDa. |
ターゲット情報
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機能
Carries out a dual function: signal transduction and activation of transcription. Involved in interleukin-4 signalling. -
配列類似性
Belongs to the transcription factor STAT family.
Contains 1 SH2 domain. -
翻訳後修飾
Tyrosine phosphorylated following stimulation by IL-4 and IL-3. -
細胞内局在
Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation. - Information by UniProt
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参照データベース
- Entrez Gene: 6778 Human
- Omim: 601512 Human
- SwissProt: P42226 Human
- Unigene: 524518 Human
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別名
- 12S1644 antibody
- D12S1644 antibody
- IL 4 STAT antibody
see all
画像
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Lanes 1-2 : STAT6 antibody
Lanes 3-4 : Anti-STAT6 (phospho Y641) antibody (ab28829) at 1/1000 dilution
Lanes 1 & 3 : K562 cells treated with 100 ng/mL IL4
Lanes 2 & 4 : K562 untreated cells
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Dylight 800 secondary antibody at 1/10000 dilution
Predicted band size: 94 kDaLoading control is Actin
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Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-STAT6 (phospho Y641) antibody (ab28829) at 1/100 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling STAT6 (phospho Y641) with ab28829. Right pane - blocked with the phospho peptide.
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ab28829 at 1/100 staining mouse lung tissue sections by IHC-P. The tissue was formaldehyde fixed and blocked with serum. A heat mediated antigen retrieval step was performed prior to incubation with the antibody. Ab6720 (Goat polyclonal to Rabbit IgG H&L (biotin)) was used as the secondary antibody.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (34)
ab28829 は 34 報の論文で使用されています。
- Yu JE et al. Anti-Chi3L1 antibody suppresses lung tumor growth and metastasis through inhibition of M2 polarization. Mol Oncol 16:2214-2234 (2022). PubMed: 34861103
- Hara T et al. CD73+ Epithelial Progenitor Cells That Contribute to Homeostasis and Renewal Are Depleted in Eosinophilic Esophagitis. Cell Mol Gastroenterol Hepatol 13:1449-1467 (2022). PubMed: 35108658
- Lin YT et al. JAK2 Phosphorylation Signals and Their Associated Cytokines Involved in Chronic Rhinosinusitis with Nasal Polyps and Correlated with Disease Severity. Biomolecules 11:N/A (2021). PubMed: 34356683
- Zhang C et al. Interleukins 4 and 13 drive lipid abnormalities in skin cells through regulation of sex steroid hormone synthesis. Proc Natl Acad Sci U S A 118:N/A (2021). PubMed: 34521750
- Huang TC et al. Thrombomodulin facilitates peripheral nerve regeneration through regulating M1/M2 switching. J Neuroinflammation 17:240 (2020). PubMed: 32799887