Anti-VEGF Receptor 1 抗体 (ab2350)
Key features and details
- Rabbit polyclonal to VEGF Receptor 1
- Suitable for: WB, IHC-P, ICC/IF, Electron Microscopy
- Reacts with: Mouse, Rat, Human, Pig
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-VEGF Receptor 1 antibody
VEGF Receptor 1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to VEGF Receptor 1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IF, Electron Microscopymore details -
種交差性
交差種: Mouse, Rat, Human, Pig -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- IHC-P: Human angiosarcoma tissue. ICC/IF: A431 cell line
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特記事項
VEGFR 1 is a cell membrane receptor kinase. It has a high affinity receptor for vascular endothelial growth factor and is putatively involved in the growth of endothelial cells and angiogenesis.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
一次抗体 備考
VEGFR 1 is a cell membrane receptor kinase. It has a high affinity receptor for vascular endothelial growth factor and is putatively involved in the growth of endothelial cells and angiogenesis. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab2350の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 151 kDa. PubMed: 21912702
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IHC-P | (2) |
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 5 µg/ml.
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Electron Microscopy |
Use at an assay dependent concentration. PubMed: 18647955
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特記事項 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 151 kDa. PubMed: 21912702 |
IHC-P
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 5 µg/ml. |
Electron Microscopy
Use at an assay dependent concentration. PubMed: 18647955 |
ターゲット情報
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機能
Receptor for VEGF, VEGFB and PGF. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. Isoform SFlt1 may have an inhibitory role in angiogenesis. -
組織特異性
Mostly in normal lung, but also in placenta, liver, kidney, heart and brain tissues. Specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. Isoform sFlt1 is strongly expressed in placenta. -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 2321 Human
- Entrez Gene: 14254 Mouse
- Entrez Gene: 54251 Rat
- Omim: 165070 Human
- SwissProt: P17948 Human
- SwissProt: P35969 Mouse
- SwissProt: P53767 Rat
- Unigene: 594454 Human
see all -
別名
- EC 2.7.10.1 antibody
- FLT 1 antibody
- FLT antibody
see all
画像
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ICC/IF image of ab2350 stained A431 (human epidermoid carcinoma cell line) cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2350, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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ab2350 - immunohistochemistry
Formalin fixed paraffin embedded human angiosarcoma stained with VEGF Receptor 1, using ABC and AEC chromogen.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (61)
ab2350 は 61 報の論文で使用されています。
- Lu C et al. Tumor necrosis factor receptor type 1-associated death domain (TRADD) regulates epithelial-mesenchymal transition (EMT), M1/M2 macrophage polarization and ectopic endometrial cysts formation in endometriosis. Ann Transl Med 9:148 (2021). PubMed: 33569450
- Qu H et al. HIF-3a affects preeclampsia development by regulating EVT growth via activation of the Flt-1/JAK/STAT signaling pathway in hypoxia. Mol Med Rep 23:N/A (2021). PubMed: 33215219
- Li J et al. A pentapeptide enabled AL3810 liposome-based glioma-targeted therapy with immune opsonic effect attenuated. Acta Pharm Sin B 11:283-299 (2021). PubMed: 33532193
- Kim SH et al. Porcine endometrial 3D co-culture: Morphological changes in 3D endometrium tissues according to hormonal changes. Histol Histopathol N/A:18335 (2021). PubMed: 33829420
- Silva JAF et al. Spatial and temporal VEGF receptor intracellular trafficking in microvascular and macrovascular endothelial cells. Sci Rep 11:17400 (2021). PubMed: 34462507